Two different tetracycline resistance mechanisms, plasmid-carried tet(L) and chromosomally located transposon-associated tet(M), coexist in Lactobacillus sakei Rits 9

Applied and Environmental Microbiology
Mohammed S AmmorAbelardo Margolles

Abstract

Lactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. sakei Rits 9, a strain isolated from Italian Sola cheese made from raw milk, is mediated by a transposon-associated tet(M) gene coding for a ribosomal protection protein and a plasmid-carried tet(L) gene coding for a tetracycline efflux pump. pLS55, the 5-kb plasmid carrying the tet(L) gene, is highly similar to the pMA67 plasmid recently described for Paenibacillus larvae, a species pathogenic to honeybees. pLS55 could be transferred by electroporation into the laboratory strain L. sakei 23K. While the L. sakei 23K transformant containing pLS55 displayed an intermediate tetracycline resistance level (MIC, <32 microg/ml), L. sakei Rits 9, containing both tetracycline-resistant determinants, had a MIC of <256 microg/ml, suggesting that Tet L and Tet M confer different levels of resistance in L. sakei. Remarkably, in the absence of tetracycline, a basal expression of both gene...Continue Reading

References

Feb 12, 1998·Nature·V PerretenM Teuber
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·S A Khan
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·G del SolarR Díaz-Orejas
Jul 14, 1998·Antimicrobial Agents and Chemotherapy·J I RossW J Cunliffe
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·I Chopra, M Roberts
Mar 20, 2002·Research in Microbiology·Marie-Christine Champomier-VergèsMonique Zagorec
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Elisabeth GrohmannManuel Espinosa
Oct 24, 2003·Current Opinion in Microbiology·Henrik C Wegener
Apr 2, 2005·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·W J Z'GraggenD Conen
Apr 13, 2005·Journal of Microbiological Methods·Angela H A M van HoekHenk J M Aarts
Apr 20, 2005·FEMS Microbiology Letters·Marilyn C Roberts
May 5, 2005·Applied and Environmental Microbiology·Odile PetsarisRoland Leclercq
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hervé TettelinClaire M Fraser
Nov 8, 2005·Nature Biotechnology·Stéphane ChaillouMonique Zagorec
Aug 1, 1993·Applied and Environmental Microbiology·D J O'sullivan, T R Klaenhammer
Apr 7, 2006·Applied and Environmental Microbiology·Monique Haarman, Jan Knol
Sep 13, 2006·FEMS Immunology and Medical Microbiology·Miguel GueimondeErika Isolauri
Sep 27, 2006·Antimicrobial Agents and Chemotherapy·Ana Belén FlórezBaltasar Mayo
Jan 18, 2007·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·K CauwertsA Decostere
Oct 25, 2007·Journal of Molecular Microbiology and Biotechnology·Mohammed Salim AmmorBaltasar Mayo
Sep 1, 2006·Meat Science·M P Doyle, M C Erickson
May 1, 1996·Microbiology·Françoise BerthierFrançoise Morel-Deville

❮ Previous
Next ❯

Citations

May 31, 2012·Antonie van Leeuwenhoek·Surya Chandra Rao Thumu, Prakash M Halami
Nov 13, 2010·Biotechnology Letters·Eun Ju Yang, Hae Choon Chang
Apr 29, 2011·Genes & Nutrition·Chiara DevirgiliisGiuditta Perozzi
Nov 9, 2010·Applied and Environmental Microbiology·Robert W PhelanTeresa M Barbosa
Apr 28, 2009·Applied and Environmental Microbiology·Lucia RizzottiSandra Torriani
Mar 23, 2011·Applied and Environmental Microbiology·Ioanna-Areti AsteriKonstantinos Papadimitriou
Mar 6, 2012·Applied and Environmental Microbiology·Yaqi YouMandy J Ward
Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Aug 24, 2012·Future Microbiology·Scott T ChanceyDavid S Stephens
Dec 17, 2009·International Journal of Food Microbiology·Roberta ComunianGiorgio Giraffa
May 26, 2009·Trends in Microbiology·Adam P Roberts, Peter Mullany
Sep 12, 2012·Journal of Applied Microbiology·P CastellanoF Toldrá
Jun 25, 2015·British Poultry Science·M DecR Urban-Chmiel
Oct 9, 2015·PLoS Biology·Richard William MeekLaura Jane Violet Piddock
Sep 8, 2017·Microorganisms·Monique Zagorec, Marie-Christine Champomier-Vergès
Oct 28, 2018·Applied and Environmental Microbiology·Ilenia CampedelliPaul W O'Toole
Jul 12, 2019·International Journal of Environmental Health Research·Fernanda Modesto TolentinoJacqueline Tanury Macruz Peresi
Dec 14, 2019·Current Microbiology·Deon P NevelingLeon M T Dicks
Feb 12, 2019·Antimicrobial Resistance and Infection Control·Yifei Zhang, Qian Zhang
Dec 12, 2018·International Journal of Microbiology·Elizaveta Anisimova, Dina Yarullina
Sep 27, 2019·Current Microbiology·Elizaveta A Anisimova, Dina R Yarullina
Jun 25, 2015·Microbiology Spectrum·Cris Fernández-LópezManuel Espinosa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.