Two-dimensional analysis of exoproteins of methicillin-resistant Staphylococcus aureus (MRSA) for possible epidemiological applications

Microbiology and Immunology
Miyo NakanoMichio Oht

Abstract

We applied two-dimensional gel electrophoresis (2-DE) to the total exoproteins secreted from pathogenic MRSA strains and identified major protein spots by N-terminal amino acid sequence analysis. In approximately 300 to 500 spots visualized on each gel, various exoproteins and cell-associated proteins were identified and their sites on the gels confirmed for construction of a reference map. Major exotoxins such as enterotoxins SEA, SEB, and SEC,, toxic shock syndrome toxin-1 (TSST-1), and hemolysins were distributed in the region of pI 6.8 to 8.1 and MW 21 to 35 kDa. Although the differences between calculated and observed values of pI and MW were relatively small in each exoprotein, those of several proteins including alpha-hemolysin and SEB were considerably deviated from the positions of the expected values. Some exoproteins were detected as multiple spots. These included beta-hemolysin, enterotoxins SEA, SEB, and SEC3, glutamic acid-specific endopeptidase, glycerophosphoryl diester phosphodiesterase and triacylglycerol lipase. The multiple spots of these exoproteins may be generated by the action of own proteases. Certain similarities of 2-DE patterns among strains belonging to the same coagulase types were observed. On the...Continue Reading

References

Mar 1, 1992·Journal of Bacteriology·J Rollof, S Normark
Dec 1, 1991·Proceedings of the Society for Experimental Biology and Medicine·H M JohnsonC H Pontzer
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1989·Journal of Bacteriology·K W Bayles, J J Iandolo
Dec 1, 1989·Journal of Medical Microbiology·J Z JordensR J Williams
Jan 1, 1989·Annual Review of Microbiology·J J Iandolo
Apr 25, 1989·Nucleic Acids Research·S J ProjanR P Novick
Mar 1, 1986·Journal of Clinical Microbiology·H IgarashiM S Bergdoll
Aug 25, 1987·Nucleic Acids Research·C Carmona, G L Gray
Jan 1, 1988·Journal of Bacteriology·M J Betley, J J Mekalanos
Jul 1, 1988·Journal of Bacteriology·J L CouchM J Betley
Apr 1, 1986·Journal of Bacteriology·C L Jones, S A Khan
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S LöfdahlM Lindberg
Nov 25, 1983·Nucleic Acids Research·T Sako, N Tsuchida
Nov 1, 1984·Infection and Immunity·G S Gray, M Kehoe
Apr 1, 1995·Applied and Environmental Microbiology·Y C Su, A C Wong
Mar 1, 1994·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·S NakaharaK Kajimura
Oct 1, 1993·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·K AraiS Yokota
Apr 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T NadaN Nakashima
Dec 24, 1997·Trends in Microbiology·B HendersonB Wren
Mar 12, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T A WichelhausV Brade
Aug 8, 1998·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·H KiyotaM Ueda
Aug 26, 1998·The New England Journal of Medicine·F D Lowy

❮ Previous
Next ❯

Citations

Jun 7, 2007·Current Microbiology·Keiko YamadaMichio Ohta
Mar 27, 2012·Journal of Bacteriology·Mark A SchallenbergerFloyd E Romesberg
Jul 21, 2005·Journal of Bacteriology·Ching Wen Tseng, George C Stewart
Jan 16, 2014·International Journal of Medical Microbiology : IJMM·Harald Kusch, Susanne Engelmann
Sep 12, 2018·Molecular & Cellular Proteomics : MCP·Tobias BuscheHaike Antelmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.