Two-Dimensional Cellular and Three-Dimensional Bio-Printed Skin Models to Screen Topical-Use Compounds for Irritation Potential

Frontiers in Bioengineering and Biotechnology
Zhengxi WeiMarc Ferrer

Abstract

Assessing skin irritation potential is critical for the safety evaluation of topical drugs and other consumer products such as cosmetics. The use of advanced cellular models, as an alternative to replace animal testing in the safety evaluation for both consumer products and ingredients, is already mandated by law in the European Union (EU) and other countries. However, there has not yet been a large-scale comparison of the effects of topical-use compounds in different cellular skin models. This study assesses the irritation potential of topical-use compounds in different cellular models of the skin that are compatible with high throughput screening (HTS) platforms. A set of 451 topical-use compounds were first tested for cytotoxic effects using two-dimensional (2D) monolayer models of primary neonatal keratinocytes and immortalized human keratinocytes. Forty-six toxic compounds identified from the initial screen with the monolayer culture systems were further tested for skin irritation potential on reconstructed human epidermis (RhE) and full thickness skin (FTS) three-dimensional (3D) tissue model constructs. Skin irritation potential of the compounds was assessed by measuring tissue viability, trans-epithelial electrical resi...Continue Reading

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Citations

Sep 17, 2020·International Journal of Molecular Sciences·Arantza Perez-ValleIsabel Andia
Jul 28, 2020·Particle and Fibre Toxicology·Irini M DijkhoffBarbara Rothen-Rutishauser
May 29, 2021·Nature Reviews. Disease Primers·Pamela L ScheinmanAri M Goldminz
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May 14, 2021·Clinical and Translational Science·Christopher P Austin
Aug 24, 2021·Colloids and Surfaces. B, Biointerfaces·Chuang GaoYuanyuan Liu

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Methods Mentioned

BETA
bioprinting
Assay
bioprinter
chemical treatment

Software Mentioned

NCATS
Leica LAS X
TEER
GraphPad Prism
DISCOVERY WORKBENCH

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