Two-dimensional chromatography of complex polymers, 8. Separation of fatty alcohol ethoxylates simultaneously by end group and chain length

Journal of Separation Science
Jacques-Antoine RaustHarald Pasch

Abstract

A comprehensive two-dimensional liquid chromatography system was developed to precisely describe the molecular heterogeneity of fatty alcohol ethoxylates. The end-group functionality was analyzed by gradient HPLC while ethylene oxide oligomer distributions were characterized by liquid adsorption chromatography. A baseline separation of all functionality fractions irrespective of the ethylene oxide oligomer chain length was achieved on nonpolar X-Terra(®) C(18) with a methanol-water gradient, whereas an isocratic flow of isopropanol-water on a polar Chromolith(®) Si column gave a separation according to the oligomer chain length without interference of the end-group distribution. The combination of these two methods to conduct online two-dimensional liquid chromatography experiments resulted in a comprehensive two-dimensional picture on the molecular heterogeneity of the sample.

References

Feb 27, 2007·Journal of Chromatography. a·J Brent Friesen, Guido F Pauli
Apr 6, 2007·Analytical Chemistry·Alain BerthodGuido F Pauli
Aug 1, 2008·Journal of Natural Products·Guido F PauliJ Brent Friesen
Sep 20, 2008·Journal of Chromatography. a·Stéphane DubantJohn Mitchell
Jan 27, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·A DesaiM L Tripp

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Citations

Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Jun 13, 2012·Journal of Separation Science·Clinton J DahlbergBrian J Carroll

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