Abstract
Photosystem II (PS II) is a photosynthetic reaction center found in higher plants which has the unique ability to evolve oxygen from water. Several groups have formed two-dimensional PS II crystals or have isolated PS II complexes and studied them by electron microscopy and image analysis. The majority of these specimens have not been well characterized biochemically and have yielded relatively low resolution two-dimensional projection maps with a variety of unit cell sizes. We report the characterization of the polypeptide and lipid content of tubular crystals of PS II. The crystals contain the reaction center core polypeptides D1, D2, cytochrome b559, as well as the chlorophyll-binding polypeptides (CP) CP47, CP43, CP29, CP26, CP24, and CP22. The lipid composition was similar to the lipids found in the stacked portion of thylakoids. We also report a 2.0-nm resolution projection map determined by electron microscopy and image analysis of frozen, hydrated PS II crystals. This projection map includes information on the portion of the complex buried in the lipid bilayer. The unit cell is a dimer with unit vectors of 17.0 and 11.4 nm separated by an angle of 106.6 degrees. In addition, Fab fragments against D1 and cytochrome b559 ...Continue Reading
References
Sep 1, 1992·The Journal of Cell Biology·D N MastronardeM E Porter
Feb 15, 1992·European Journal of Biochemistry·R Bassi, P Dainese
Apr 20, 1990·European Journal of Biochemistry·E HaagG Renger
Apr 3, 1990·Biochemistry·J P DekkerE J Boekema
Nov 21, 1986·Cell·R T SayreL Bogorad
Dec 5, 1988·FEBS Letters·M Ikeuchi, Y Inoue
Dec 27, 1988·Biochemistry·G S TaeL Bogorad
Dec 1, 1988·European Journal of Biochemistry·K D IrrgangG Renger
Nov 1, 1987·The Journal of Cell Biology·M SeibertL A Staehelin
Aug 1, 1986·European Journal of Biochemistry·U LjungbergB Andersson
Jul 1, 1987·The Journal of Cell Biology·E KubalekN Unwin
Oct 1, 1986·The Journal of Cell Biology·J S Jacob, K R Miller
Jun 6, 1985·Nature·A Brisson, P N Unwin
Dec 11, 1984·Nucleic Acids Research·K HolschuhP R Whitfeld
Jan 1, 1984·Ultramicroscopy·R A MilliganP N Unwin
May 1, 1980·Journal of Biochemical and Biophysical Methods·A Rawyler, P A Siegenthaler
Aug 1, 1982·Journal of Microscopy·W O Saxton, W Baumeister
Jan 1, 1995·Journal of Structural Biology·X W GuoC A Mannella
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J BoekemaM Rögner
Feb 1, 1994·Journal of Bioenergetics and Biomembranes·E J BoekemaM Rögner
Feb 17, 1994·Nature·W KühlbrandtY Fujiyoshi
Apr 1, 1994·European Journal of Biochemistry·C SantiniR Bassi
Dec 1, 1993·The Journal of Cell Biology·D N MastronardeJ R McIntosh
Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson
Jul 5, 1993·Biochimica Et Biophysica Acta·E M AroB Andersson
Mar 1, 1993·Journal of Structural Biology·M K LyonP S Furcinitti
Feb 1, 1993·Ultramicroscopy·Z H Zhou, W Chiu
Citations
Nov 1, 1996·Photosynthesis Research·G TsiotisD Ghanotakis
Aug 1, 1996·Photosynthesis Research·D BaldM Rögner
Aug 1, 1997·Current Opinion in Structural Biology·J B HeymannA Engel
Aug 17, 1999·Current Opinion in Structural Biology·J Barber, W Kühlbrandt
Jan 23, 1999·European Journal of Biochemistry·G TsiotisD Ghanotakis
Jan 15, 1997·European Journal of Biochemistry·B HankamerJ Barber
Apr 1, 1997·Photochemistry and Photobiology·R A UphausM Seibert
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Ben HankamerEgbert J. Boekema
Jun 19, 1998·Biochimica Et Biophysica Acta·M K Lyon
Jun 1, 2011·Analytical Biochemistry·Siok Wan GanJaume Torres
Nov 7, 1998·Biochimica Et Biophysica Acta·P B Lukins, T Oates
Jun 15, 1997·Structure·E P MorrisJ Barber
Nov 25, 1998·Biophysical Journal·J GallantC Salesse
Jun 26, 2001·Biophysical Journal·E TrudelC Salesse
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai
Jan 27, 1998·Journal of Structural Biology·D N Mastronarde
Jun 10, 1998·Journal of Structural Biology·T Walz, N Grigorieff
Jun 10, 1998·Journal of Structural Biology·S A Müller, A Engel
Jan 8, 1999·Journal of Structural Biology·K MayanagiK Nakazato
Aug 1, 1997·Journal of Structural Biology·L HaslerG Tsiotis
Feb 29, 2000·The Journal of Biological Chemistry·O KruseJ Barber
Dec 22, 1999·European Journal of Biochemistry·S StoylovaA Holzenburg