PMID: 8603915Mar 1, 1996Paper

Two-dimensional crystals of photosystem II: biochemical characterization, cryoelectron microscopy and localization of the D1 and cytochrome b559 polypeptides

The Journal of Cell Biology
K M MarrM K Lyon

Abstract

Photosystem II (PS II) is a photosynthetic reaction center found in higher plants which has the unique ability to evolve oxygen from water. Several groups have formed two-dimensional PS II crystals or have isolated PS II complexes and studied them by electron microscopy and image analysis. The majority of these specimens have not been well characterized biochemically and have yielded relatively low resolution two-dimensional projection maps with a variety of unit cell sizes. We report the characterization of the polypeptide and lipid content of tubular crystals of PS II. The crystals contain the reaction center core polypeptides D1, D2, cytochrome b559, as well as the chlorophyll-binding polypeptides (CP) CP47, CP43, CP29, CP26, CP24, and CP22. The lipid composition was similar to the lipids found in the stacked portion of thylakoids. We also report a 2.0-nm resolution projection map determined by electron microscopy and image analysis of frozen, hydrated PS II crystals. This projection map includes information on the portion of the complex buried in the lipid bilayer. The unit cell is a dimer with unit vectors of 17.0 and 11.4 nm separated by an angle of 106.6 degrees. In addition, Fab fragments against D1 and cytochrome b559 ...Continue Reading

References

Sep 1, 1992·The Journal of Cell Biology·D N MastronardeM E Porter
Feb 15, 1992·European Journal of Biochemistry·R Bassi, P Dainese
Dec 1, 1988·European Journal of Biochemistry·K D IrrgangG Renger
Aug 1, 1986·European Journal of Biochemistry·U LjungbergB Andersson
Oct 1, 1986·The Journal of Cell Biology·J S Jacob, K R Miller
Jun 6, 1985·Nature·A Brisson, P N Unwin
May 1, 1980·Journal of Biochemical and Biophysical Methods·A Rawyler, P A Siegenthaler
Aug 1, 1982·Journal of Microscopy·W O Saxton, W Baumeister
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J BoekemaM Rögner
Dec 1, 1993·The Journal of Cell Biology·D N MastronardeJ R McIntosh
Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson
Jul 5, 1993·Biochimica Et Biophysica Acta·E M AroB Andersson
Mar 1, 1993·Journal of Structural Biology·M K LyonP S Furcinitti

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Citations

Nov 1, 1996·Photosynthesis Research·G TsiotisD Ghanotakis
Aug 1, 1997·Current Opinion in Structural Biology·J B HeymannA Engel
Aug 17, 1999·Current Opinion in Structural Biology·J Barber, W Kühlbrandt
Jan 23, 1999·European Journal of Biochemistry·G TsiotisD Ghanotakis
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Ben HankamerEgbert J. Boekema
Jun 19, 1998·Biochimica Et Biophysica Acta·M K Lyon
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai
Jan 27, 1998·Journal of Structural Biology·D N Mastronarde
Jun 10, 1998·Journal of Structural Biology·T Walz, N Grigorieff
Jan 8, 1999·Journal of Structural Biology·K MayanagiK Nakazato
Feb 29, 2000·The Journal of Biological Chemistry·O KruseJ Barber

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