Two-Dimensional Gel Electrophoresis Analysis of the Response of Pseudomonas putida KT2442 to 2-Chlorophenol.

Applied and Environmental Microbiology
C G LupiC A Mason

Abstract

The effects of exposure of Pseudomonas putida KT2442 to 2-chlorophenol as a model for the chemical stress response were examined by two-dimensional polyacrylamide gel electrophoresis. Individual protein concentrations were determined at 45, 65, and 95 min following the addition of 2-chlorophenol at a concentration of 1.63 mM to exponentially growing cultures of P. putida KT2442 by silver staining the separated proteins. The changes in the protein concentrations could be classified into four categories, namely those which increased continuously during exposure, those which decreased in concentration, those which showed a concentration peak at some point following exposure, and those which were essentially unaffected. Thirty proteins with isoelectric points between pH 4 and 6 increased in concentration, 27 decreased, and 90 had a concentration maximum or minimum between 45 and 95 min. Of those proteins with isoelectric points between 5.5 and 10, 68 increased in concentration, 39 decreased in concentration, and 47 showed a concentration peak in the middle of the sampling period. Thus, in the evaluation of the stress response, a functional description requires an understanding both of proteins which are required at higher concentra...Continue Reading

References

Oct 1, 1977·Analytical Biochemistry·V F Kalb, R W Bernlohr
Jul 1, 1979·Journal of Bacteriology·S L HerendeenF C Neidhardt
Dec 1, 1992·Electrophoresis·R A VanBogelenF C Neidhardt
Jan 1, 1992·Journal of Biotechnology·A HeitzerG Hamer
Jan 1, 1992·Applied and Environmental Microbiology·A BlomA Matin
Jun 1, 1991·Ecotoxicology and Environmental Safety·C Odberg-FerragutD Dive
Oct 1, 1990·Journal of Bacteriology·H E Kroh, L D Simon
Jan 1, 1993·Annual Review of Microbiology·T YuraH Mori
Nov 1, 1993·Journal of Bacteriology·T J WelchD H Bartlett
Jan 1, 1993·Annual Review of Cell Biology·C Georgopoulos, W J Welch
Feb 1, 1993·Biochemical Society Transactions·A Görg

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Citations

Jan 1, 1997·Journal of Basic Microbiology·D BenndorfW Babel
Mar 13, 2009·Briefings in Functional Genomics & Proteomics·Carla M R Lacerda, Kenneth F Reardon
Mar 23, 2000·Letters in Applied Microbiology·A J DuncanN G Love
Feb 25, 2017·Frontiers in Plant Science·Kamlesh K MeenaParamjit S Minhas
May 1, 1997·Applied and Environmental Microbiology·P Vercellone-Smith, D S Herson

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