Two-dimensional gel electrophoresis analysis of endovacuolar organelles

Electrophoresis
S ScianimanicoMichel Desjardins

Abstract

Cells perform their multiple functions with the aid of a series of distinct membrane organelles. In the last years, many of these compartments have been isolated, purified, and extensively studied. The major roles of each organelle in the cell are well understood. However, most of the molecular basis by which they perform their functions is poorly known. The recent identification and study of a handful of proteins associated with endovacuolar compartments has had a major impact on the understanding of the molecular details of organelle functions even though two-dimensional (2-D) gel analysis indicates that hundreds of proteins are typically associated with a complex organelle. This shows that many details and surprises are still to come for cell biologists. In the present study, we have analyzed and compared different organelles of the endocytic and phagocytic apparatus using 2-D gel electrophoresis.

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Citations

Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Feb 26, 1999·The Plant Journal : for Cell and Molecular Biology·V SantoniM Rossignol
Feb 24, 2001·Cellular Microbiology·S Duclos, M Desjardins
Jan 10, 2001·The Journal of Cell Biology·J GarinM Desjardins
Feb 24, 2001·Traffic·W H McDonald, J R Yates

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