Two-dimensional gel electrophoresis-based analysis provides global insights into the cotton ovule and fiber proteomes

Science China. Life Sciences
Xiang JinXuchu Wang

Abstract

Proteomic analysis of upland cotton was performed to profile the global detectable proteomes of ovules and fibers using two-dimensional electrophoresis (2DE). A total of 1,203 independent protein spots were collected from representative 2DE gels, which were digested with trypsin and identified by matrix-assisted laser desorption and ionization-time-offlight/ time-of-flight (MALDI-TOF/TOF) mass spectrometry. The mass spectrometry or tandem mass spectrometry (MS or MS/MS) data were then searched against a local database constructed from Gossypium hirsutum genome sequences, resulting in successful identification of 975 protein spots (411 for ovules and 564 for fibers). Functional annotation analysis of the 975 identified proteins revealed that ovule-specific proteins were mainly enriched in functions related to fatty acid elongation, sulfur amino acid metabolism and post-replication repair, while fiber-specific proteins were enriched in functions related to root hair elongation, galactose metabolism and D-xylose metabolic processes. Further annotation analysis of the most abundant protein spots showed that 28.96% of the total proteins in the ovule were mainly located in the Golgi apparatus, endoplasmic reticulum, mitochondrion and...Continue Reading

References

Sep 5, 2002·Biochemical and Biophysical Research Communications·Shengjian JiYuxian Zhu
Sep 12, 2006·BMC Genomics·Fiona M McCarthyShane C Burgess
May 20, 2008·Nucleic Acids Research·Qi Zheng, Xiu-Jie Wang
Oct 1, 2008·Journal of Proteome Research·Yi-Wei YangJin-Yuan Liu
Nov 27, 2008·Bioinformatics·Seth CarbonUNKNOWN Web Presence Working Group
Oct 15, 2010·Current Opinion in Plant Biology·Yong-Mei Qin, Yu-Xian Zhu
Oct 28, 2011·Journal of Proteome Research·Yanyan MengShuxun Yu
Jan 31, 2012·Plant Physiology and Biochemistry : PPB·Farah DeebaVivek Pandey
Feb 14, 2012·Plant Science : an International Journal of Experimental Plant Biology·Fu'an ZhaoShuping Lv
Aug 28, 2012·Nature Genetics·Kunbo WangShuxun Yu
Mar 26, 2013·Science China. Life Sciences·Fuchu He
May 23, 2013·Journal of Integrative Plant Biology·Changsong ZouGuoli Song
May 20, 2014·Nature Genetics·Fuguang LiShuxun Yu
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
May 2, 2015·Science China. Life Sciences·XiaoFeng Cao

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Citations

Jan 25, 2016·Science China. Life Sciences·Yuxian Zhu
Aug 2, 2019·Biotechnology for Biofuels·Ho Myeong KimHae Woong Park

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