PMID: 6972714Jan 1, 1980Paper

Two-dimensional gel electrophoresis of cattle plasma proteins: genetic polymorphism of an alpha 1-protease inhibitor

Animal Blood Groups and Biochemical Genetics
R K Juneja, B Gahne

Abstract

Two-dimensional electrophoretic analysis of cattle plasma proteins was done by a first dimension separation in agarose gel (pH 5.0), followed by a second dimension in horizontal polyacrylamide gel (pH 9.0). This method resulted in improved and reproducible separation of many alpha- and beta-globulins. Three groups of alpha-globulins, designated Pi-1, Pi-2 and Pi-3, were found to inhibit the esterolytic activity of bovine trypsin and bovine chymotrypsin. Pi-2 showed appreciable inhibition only for trypsin and genetic polymorphism was observed for this protein. Family data supported the hypothesis that the three Pi-2 types observed were controlled by two codominant, autosomal alleles. The occurrence of a third Pi-2 allele was also postulated in some animals studied. The frequency of the most common allele, Pi-2s, ranged from 0.5-0.8 in the different breeds of cattle studied (Swedish Red and White, Friesian, Jersey, Charolais and Simmental). The post-transferrins Ptf-1 and Ptf-2 in cattle plasma were shown to be two different genetic systems.

References

Jan 1, 1978·Animal Blood Groups and Biochemical Genetics·R K JunejaK Sandberg
Jan 1, 1979·Animal Blood Groups and Biochemical Genetics·R K JunejaK Sandberg
Jun 10, 1977·Biochimica Et Biophysica Acta·J S BaumstarkR J Luby
Aug 15, 1977·European Journal of Biochemistry·B NordenmanI Björk
Jan 1, 1978·Animal Blood Groups and Biochemical Genetics·B Gahne, R K Juneja
Jan 1, 1976·Animal Blood Groups and Biochemical Genetics·F ThinnesU Wens
Jun 28, 1968·Annals of the New York Academy of Sciences·E Habermann
Jan 22, 1968·Biochimica Et Biophysica Acta·J P Binette

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Citations

Jan 1, 1983·Animal Blood Groups and Biochemical Genetics·R K JunejaE Andresen
Oct 17, 2009·Proteomics·Ingrid MillerElisabetta Gianazza
Nov 1, 1988·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·L KaartinenM Sandholm
Jan 1, 1986·Animal Genetics·A L Archibald, J Webster
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S D Patterson
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A DubinA Koj

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