Two-dimensional lectin affinity electrophoresis of alpha-fetoprotein: characterization of erythroagglutinating phytohemagglutinin-dependent microheterogeneity forms.

Electrophoresis
K TaketaH Hirai

Abstract

By means of two-dimensional lectin affinity electrophoresis of human alpha-fetoprotein (AFP) from different sources, AFP bands separated with erythroagglutinating phytohemagglutinin (E-PHA) were further characterized with other lectins of known oligosaccharide specificities. The results with a cord serum AFP revealed that not only AFP-P2 (E-PHA-nonreactive) but also AFP-P4 and P5 (E-PHA-reactive) had affinities for Concanavalin A (Con A) and Allomyrina dichotoma lectin (allo A), indicating that the cord serum AFP has nonbisected biantennary complex-type oligosaccharides with the terminal galactose on Man alpha 1----6 residue sialylated at the C-6, but not C-3, position. On the other hand, the results with a hepatoblastoma (HUH-6 C1-5 cell line) AFP showed that not only AFP-P5 but also AFP-P1 (E-PHA-nonreactive) and P3 (E-PHA-less reactive) had Con A-nonreactive AFP and that AFP-P1 had AFP-A1 (allo A-nonreactive) and AFP-A2 (allo A-less reactive), and AFP-P3 and P4 had AFP-A1s (allo A-nonreactive), as main components, in addition to the spots of cord serum AFP. Most of the E-PHA-dependent bands of AFP were further subdivided with Lens culinaris agglutinin (LCA-A) into LCA-A-reactive, weakly reactive and nonreactive spots. Simila...Continue Reading

References

Aug 23, 1985·Biochimica Et Biophysica Acta·Y AoyagiF Ichida
Jul 1, 1981·Scandinavian Journal of Immunology·J BreborowiczD Breborowicz

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Citations

Jun 1, 1991·Electrophoresis·D SchranzG I Abelev
Oct 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·K ShimizuS Matsuura
Jun 10, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Evi N Debruyne, Joris R Delanghe
Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Dec 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Taketa
May 16, 2008·Journal of Proteome Research·Takatoshi NakagawaAkihiro Kondo
Sep 13, 1991·Journal of Chromatography·K Taketa

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