Two-dimensional NMR studies of the interactions between a peptide of cholera toxin and monoclonal antibodies

Biopolymers
J AnglisterR Levy

Abstract

To increase our understanding of the molecular basis for antibody specificity and for the cross-reactivity of antipeptide antibodies with native proteins, it is important to study the three-dimensional structure of antibody complexes with their peptide antigens. For this purpose it may not be necessary to solve the structure of the whole antibody complex but rather to concentrate on elucidating the combining site structure, the interactions of the antibody with its antigen, and the bound peptide conformation. To extract the information about antibody-peptide interactions and intramolecular interactions in the bound ligand from the complicated and unresolved spectrum of the Fab-peptide complex (Fab: antibody fragment made of Fv--the antibody fragment composed of the variable regions of the light and heavy chains forming a single combining site for the antigen--the light chain, and the first heavy chain constant regions), an nmr methodology based on measurements of two-dimensional transferred nuclear Overhauser effect (NOE) difference spectra was developed. Using this methodology the interactions of three monoclonal antibodies with a cholera toxin peptide were studied. The observed interactions were assigned to the antibody proto...Continue Reading

References

Oct 30, 1990·Biochemistry·B ZilberJ Anglister
Dec 21, 1989·Nature·C ChothiaW R Tulip
Aug 20, 1987·Journal of Molecular Biology·C Chothia, A M Lesk
Dec 31, 1973·Annals of the New York Academy of Sciences·A A Bothner-By, R Gassend
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C O JacobR Arnon
Aug 20, 1993·Journal of Molecular Biology·M Shoham
Jan 1, 1987·Immunology Today·M W Steward, C R Howard

❮ Previous
Next ❯

Citations

Sep 23, 1997·Protein Science : a Publication of the Protein Society·J A HubbardC M Dobson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.