Two-dimensional nuclear magnetic resonance spectroscopy with a microfluidic diamond quantum sensor

Science Advances
J SmitsV M Acosta

Abstract

Quantum sensors based on nitrogen-vacancy centers in diamond have emerged as a promising detection modality for nuclear magnetic resonance (NMR) spectroscopy owing to their micrometer-scale detection volume and noninductive-based detection. A remaining challenge is to realize sufficiently high spectral resolution and concentration sensitivity for multidimensional NMR analysis of picoliter sample volumes. Here, we address this challenge by spatially separating the polarization and detection phases of the experiment in a microfluidic platform. We realize a spectral resolution of 0.65 ± 0.05 Hz, an order-of-magnitude improvement over previous diamond NMR studies. We use the platform to perform two-dimensional correlation spectroscopy of liquid analytes within an effective ∼40-picoliter detection volume. The use of diamond quantum sensors as in-line microfluidic NMR detectors is a major step toward applications in mass-limited chemical analysis and single-cell biology.

References

Nov 14, 1997·IEEE Transactions on Bio-medical Engineering·J E StockerR L Magin
Jul 25, 2006·Journal of Magnetic Resonance·Jeremy N RobinsonPaul T Callaghan
Jul 14, 2007·Journal of Magnetic Resonance·Andrew F McDowell, Natalie L Adolphi
Feb 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·F VerpillatA Pines
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·M P LedbetterA Pines
Jul 6, 2010·Journal of Magnetic Resonance·H HarckenL Trahms
Nov 3, 2010·The Review of Scientific Instruments·Shu-Hsien LiaoShieh-Yueh Yang
Dec 21, 2010·Journal of Magnetic Resonance·H J HogbenIlya Kuprov
Jan 12, 2011·Applied Physics Letters·P SiyushevJ Wrachtrup
Mar 17, 2011·Physical Review Letters·G de LangeR Hanson
Jul 3, 2015·Angewandte Chemie·Jan-Henrik Ardenkjaer-LarsenLucio Frydman
Oct 13, 2015·Nature Communications·T StaudacherJ Wrachtrup
Apr 19, 2016·Quarterly Reviews of Biophysics·Michael KovermannMagnus Wolf-Watz
Sep 1, 2016·Current Opinion in Biotechnology·John L MarkleyDavid S Wishart
Mar 21, 2017·Scientific Reports·Marco GrisiGiovanni Boero
May 27, 2017·Science·J M BossC L Degen
Jun 3, 2017·Science·Nabeel AslamJörg Wrachtrup
Aug 5, 2017·Nature Communications·P KehayiasV M Acosta
Oct 2, 2017·The Review of Scientific Instruments·Michael C D TaylerDmitry Budker
Nov 25, 2017·The Analyst·I FugariuA J Simpson
Mar 16, 2018·Nature·David R GlennRonald L Walsworth
Mar 30, 2018·Nature Communications·David A BroadwayLloyd C L Hollenberg

❮ Previous
Next ❯

Citations

Aug 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jacob HenshawCarlos A Meriles
Apr 29, 2020·Annual Review of Biomedical Engineering·Elishai Ezra Tsur
Dec 7, 2019·Beilstein Journal of Nanotechnology·Alberto BorettiStefania Castelletto
Oct 30, 2020·Physical Review Research·Ilja FescenkoVictor M Acosta
Dec 3, 2020·The Review of Scientific Instruments·K HerbC L Degen
Apr 17, 2020·Journal of Magnetic Resonance·John W BlanchardDmitry Budker
Jun 25, 2021·Chemical Science·Sergey KorchakStefan Glöggler
Jun 13, 2020·Analytical Chemistry·Nergiz Sahin SolmazGiovanni Boero
Sep 4, 2021·Physical Review Letters·Utkarsh Mishra, Abolfazl Bayat

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
chip
chips

Software Mentioned

SPINACH

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.