Two-Dimensional Polyacrylamide Gel Electrophoresis Analysis of the Acid Tolerance Response in Listeria monocytogenes LO28.

Applied and Environmental Microbiology
B O'DriscollC Hill

Abstract

Listeria monocytogenes is capable of withstanding low pH after initial exposure to sublethal acidic conditions, a phenomenon termed the acid tolerance response (B. O'Driscoll, C. G. M. Gahan, and C. Hill, Appl. Environ. Microbiol. 62:1693-1698, 1996). Treatment of L. monocytogenes LO28 with chloramphenicol during acid adaptation abrogated the protective effect, suggesting that de novo protein synthesis is required for the acid tolerance response. Analysis of protein expression during acid adaptation by two-dimensional gel electrophoresis revealed changes in the levels of 53 proteins. Significant protein differences were also evident between nonadapted L. monocytogenes LO28 and a constitutively acid-tolerant mutant, ATM56. In addition, the analysis[S_TABC] revealed differences in protein expression between cells induced with a weak acid (lactic acid) and those induced with a strong acid (HCl). Comparison of both acid-adapted LO28 and ATM56 revealed that both are capable of maintaining their internal pH (pH(infi)) at higher levels than nonadapted control cells during severe acid stress. Collectively, the data demonstrate the profound alterations in protein synthesis which take place during acid adaptation in L. monocytogenes and ...Continue Reading

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Citations

May 7, 2003·Applied and Environmental Microbiology·Adriana FerreiraKathryn J Boor
Apr 7, 2006·Applied and Environmental Microbiology·Marcelo BonnetThomas J Montville
Oct 3, 2009·Journal of Applied Microbiology·L WaddingtonM Kalmokoff
Jan 22, 2003·Journal of Applied Microbiology·J K LeeW M Waites
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Paul D Cotter, Colin Hill
Mar 5, 2013·Archives of Microbiology·Jessie MeloMaria Leonor Faleiro
Jun 29, 2001·Microbiology·M De AngelisM Gobbetti
Mar 2, 2002·Journal of Applied Microbiology·N Browne, B C A Dowds
Jan 20, 2004·Proteomics·Maria De Angelis, Marco Gobbetti
Jan 1, 2004·Comprehensive Reviews in Food Science and Food Safety·N Beales
May 14, 1999·Current Opinion in Microbiology·J W Foster
Oct 4, 2000·International Journal of Food Microbiology·P D CotterC Hill

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