Two dimensional separations of human urinary protein digest using a droplet-interfaced platform

Analytica Chimica Acta
Linquan YeBo Zhang

Abstract

For highly complex mixtures, coelution is a common phenomenon in chromatography. A great deal of resolution is hidden in coelution, and lost due to inevitable molecular diffusion during sample transfer. The molecular diffusion may lead to band broadening and remix of separated peaks, which cause degradation of achievable resolution. In this study, we introduced droplet microfluidics as a high performance sample transfer tool in two dimensional nanoflow liquid chromatography-capillary electrophoresis separation of a human urine sample. The fine fractionation capability and sampling completeness enabled by the droplet-interface demonstrated the 2D system's usefulness in high-resolution mapping of real world biological samples.

References

Oct 1, 1984·Analytical Chemistry·J C Giddings
Mar 10, 2001·Nature·A Abbott
Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
Mar 14, 2003·Nature·Ruedi Aebersold, Matthias Mann
Nov 7, 2006·Angewandte Chemie·Helen SongRustem F Ismagilov
Aug 1, 2008·Molecular & Cellular Proteomics : MCP·Stéphane DecramerJoost P Schanstra
Jan 15, 2009·Angewandte Chemie·J Scott EdgarDaniel T Chiu
Jun 11, 2009·Analytical Chemistry·Daniel T ChiuGavin D M Jeffries
Oct 15, 2009·Chemical Communications : Chem Comm·X Z NiuA J deMello
Jul 10, 2008·Proteomics. Clinical Applications·Joshua J CoonPhilippe Schmitt-Kopplin
Jun 24, 2010·Angewandte Chemie·Ashleigh B ThebergeWilhelm T S Huck
Oct 27, 2010·Expert Review of Proteomics·Mingxia GaoXiangmin Zhang
Apr 20, 2011·Journal of Proteome Research·Arivusudar MarimuthuAkhilesh Pandey
Apr 3, 2012·Expert Review of Proteomics·Xin XuZ Hugh Fan
Jun 27, 2012·Journal of Separation Science·Zhiliang XiaoBo Zhang
Nov 28, 2012·Angewandte Chemie·Haakan N Joensson, Helene Andersson Svahn

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Citations

Feb 28, 2020·International Journal of Molecular Sciences·Van-An DuongHookeun Lee
Dec 8, 2017·The Analyst·I Rodríguez-RuizJ A Gavira
Jul 22, 2018·Analytical and Bioanalytical Chemistry·Andrea BeutnerFrank-Michael Matysik

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