Two-dimensional separations with electrospray ionization ambient pressure high-resolution ion mobility spectrometry/quadrupole mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Laura M MatzHerbert H Hill

Abstract

The coupling of ion mobility spectrometry (IMS) instruments with mass spectrometers has been described since early in IMS development, most commonly with quadrupole mass analyzers. The recent development of IMS with time-of-flight (TOF) instruments has demonstrated that the time compatibility (IMS milliseconds and TOFMS microseconds) of the two techniques enables rapid two-dimensional separations to be performed, theoretically in the order of seconds for a complete analysis. This study presents a unique way to operate a traditional IMS/QMS system to attain separations similar to those achieved with IMS/TOF. For this new approach, the quadrupole was slowly scanned in the single-ion monitoring mode while IMS spectra were continually embedded in each m/z step. In this way, two-dimensional separations (IMS drift times and m/z) were obtained using the traditional IMS/QMS arrangement. An example of a five amino acid separation (quadrupole scan of 40 m/z values at a rate of approximately 7 steps/min) led to a complete two-dimensional analysis within 6 min, comparable to rapid chromatographic separations with mass spectrometry. Proposed approaches to reduce the analysis time are discussed and a reduction in the analysis time to less th...Continue Reading

References

Jun 13, 1998·Analytical Chemistry·C S HoaglundD E Clemmer
Aug 6, 1998·Journal of the American Society for Mass Spectrometry·A E CountermanD E Clemmer
Oct 30, 1998·Journal of the American Society for Mass Spectrometry·S J ValentineD E Clemmer
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Jul 1, 1997·Analytical Chemistry·Y Liu, D E Clemmer

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Citations

Feb 7, 2009·Journal of the American Society for Mass Spectrometry·Ruwan T KurulugamaDavid E Clemmer
Nov 16, 2010·Veterinary Immunology and Immunopathology·John D Lippolis, Timothy A Reinhardt
Feb 24, 2007·Rapid Communications in Mass Spectrometry : RCM·Xiaoting TangHerbert H Hill
Sep 23, 2010·Proteomics·Andrea Armirotti, Gianluca Damonte
Jan 27, 2012·Rapid Communications in Mass Spectrometry : RCM·Christopher M BentonDonald J L Jones
Jul 12, 2002·Journal of Mass Spectrometry : JMS

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