Two-dimensional tracking of ncd motility by back focal plane interferometry

Biophysical Journal
M W AllersmaC F Schmidt

Abstract

A technique for detecting the displacement of micron-sized optically trapped probes using far-field interference is introduced, theoretically explained, and used to study the motility of the ncd motor protein. Bead motions in the focal plane relative to the optical trap were detected by measuring laser intensity shifts in the back-focal plane of the microscope condenser by projection on a quadrant diode. This detection method is two-dimensional, largely independent of the position of the trap in the field of view and has approximately 10-micros time resolution. The high resolution makes it possible to apply spectral analysis to measure dynamic parameters such as local viscosity and attachment compliance. A simple quantitative theory for back-focal-plane detection was derived that shows that the laser intensity shifts are caused primarily by a far-field interference effect. The theory predicts the detector response to bead displacement, without adjustable parameters, with good accuracy. To demonstrate the potential of the method, the ATP-dependent motility of ncd, a kinesin-related motor protein, was observed with an in vitro bead assay. A fusion protein consisting of truncated ncd (amino acids 195-685) fused with glutathione-S-...Continue Reading

References

Nov 9, 1989·Nature·J HowardR D Vale
Jan 1, 1982·Methods in Enzymology·R C Williams, J C Lee
Dec 8, 1995·Science·H YinJ Gelles
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·E Meyhöfer, J Howard
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·K Svoboda, S M Block
Jun 1, 1993·The Journal of Cell Biology·S RayJ Howard
Jun 3, 1994·Cell·K Svoboda, S M Block
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·P VanBurenD M Warshaw
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R J StewartL S Goldstein
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C M CoppinR D Vale
Apr 1, 1996·Biophysical Journal·R M SimmonsJ A Spudich
Mar 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J D Moore, S A Endow
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·H HiguchiT Yanagida
Mar 1, 1997·Biophysical Journal·M D WangS M Block
Sep 4, 1997·Nature·U Henningsen, M Schliwa
Nov 14, 1997·Methods in Cell Biology·F Gittes, C F Schmidt
Oct 1, 1993·Optics Letters·L P Ghislain, W W Webb
Mar 1, 1973·Applied Optics·G M Hale, M R Querry

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Citations

Mar 3, 2007·European Biophysics Journal : EBJ·Mikhail J KorneevChristoph F Schmidt
Apr 19, 2005·Biomedical Microdevices·Henry T Schek, Alan J Hunt
Feb 27, 1999·Current Opinion in Biotechnology·M D Wang
Nov 13, 2007·Nature Methods·Maarten C NoomGijs J L Wuite
Aug 1, 2006·The Review of Scientific Instruments·Keir C Neuman, Steven M Block
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gary J Brouhard, Alan J Hunt
Jun 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bin Guo, William H Guilford
Mar 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Paige M ShakleeMarileen Dogterom
Feb 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jeneva A LaibWilliam H Guilford
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rustem I LitvinovJohn W Weisel
Apr 2, 2013·Nucleic Acids Research·Francesca FarinaGiovanni Cappello
Feb 10, 2012·Journal of the Royal Society, Interface·Nadja NijenhuisChristoph F Schmidt
Mar 1, 2008·Annual Review of Biochemistry·Jeffrey R MoffittCarlos Bustamante
Feb 27, 2009·PloS One·Sarah AdioGünther Woehlke
Jun 30, 2010·PloS One·Alicia M EvangelistaWilliam H Guilford
Apr 20, 2012·PloS One·Teuta Pilizota, Joshua W Shaevitz
Dec 3, 2013·The Review of Scientific Instruments·Kai BodensiekIwan A T Schaap
May 2, 2008·The Review of Scientific Instruments·M AtakhorramiC F Schmidt
Jul 8, 2008·The Review of Scientific Instruments·Robert WalderMichael Dennin
Feb 7, 2014·Journal of Muscle Research and Cell Motility·Amy M Clobes, William H Guilford
May 11, 2011·Physical Chemistry Chemical Physics : PCCP·Laurence G Wilson, Wilson C K Poon
Jun 24, 2010·Biochemical and Biophysical Research Communications·Johanne L PythonWilliam H Guilford
Apr 7, 2009·Micron : the International Research and Review Journal for Microscopy·L Y PozzoC L Cesar
Nov 25, 2014·Biophysical Journal·Teuta Pilizota, Joshua W Shaevitz
Nov 6, 2008·Cell Motility and the Cytoskeleton·Vijay S RaoWilliam H Guilford
Dec 11, 2007·Biophysical Journal·M TassieriT A Waigh
Mar 11, 2010·IEEE Transactions on Nanobioscience·P ThévenazM Unser
Mar 29, 2000·Biophysical Journal·S YamadaS C Kuo
Dec 16, 2010·Biophysical Journal·Anthony E ButterfieldMikhail Skliar
Aug 9, 2007·Current Biology : CB·Henry T SchekAlan J Hunt
Sep 21, 2013·Biophysical Journal·Marco Capitanio, Francesco S Pavone
Dec 26, 2003·Biophysical Journal·Linda J RinkoWilliam H Guilford
Dec 6, 2005·Biophysical Journal·Yasuharu TakagiHenry Shuman
Jun 14, 2005·Biophysical Journal·Francesco VanziYale E Goldman
Jul 25, 2006·Biophysical Journal·Gregor KnönerHalina Rubinsztein-Dunlop
Jun 25, 2002·Biophysical Journal·Matthew J LangSteven M Block
Sep 22, 2001·Biophysical Journal·M K CheezumW H Guilford
Feb 28, 2002·Biophysical Journal·U BockelmannF Heslot
Jan 28, 2003·Biophysical Journal·Erwin J G PetermanChristoph F Schmidt

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