Two discrete regions of interleukin-2 (IL2) receptor beta independently mediate IL2 activation of a PD98059/rapamycin/wortmannin-insensitive Stat5a/b serine kinase.

The Journal of Biological Chemistry
Robert A KirkenW L Farrar

Abstract

Many cytokines, hormones, and growth factors activate Janus kinases to tyrosine phosphorylate select members of the Stat transcription factors. For full transcriptional activation, Stat1 and Stat3 also require phosphorylation of a conserved serine residue within a mitogen-activated protein kinase phosphorylation consensus site. On the other hand, two recently identified and highly homologous Stat5a and Stat5b proteins lack this putative mitogen-activated protein kinase phosphorylation site. The present study set out to establish whether Stat5a and Stat5b are under the control of an interleukin-2 (IL2)-activated Stat5 serine kinase. We now report that IL2 stimulated marked phosphorylation of serine and tyrosine residues of both Stat5a and Stat5b in human T lymphocytes and in several IL2-responsive lymphocytic cell lines. No Stat5a/b phosphothreonine was detected. Phosphoamino acid analysis also revealed that Stat5a/b phosphotyrosine levels were maximized within 1-5 min of IL2 stimulation, whereas serine phosphorylation kinetics were slower. Interestingly, IL2-induced serine phosphorylation of Stat5a differed quantitatively and temporally from that of Stat5b with Stat5a serine phosphorylation leveling off after 10 min and the mor...Continue Reading

References

Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·S L GaffenW C Greene
Jan 1, 1995·Annual Review of Biochemistry·C Schindler, J E Darnell
Jun 1, 1995·Molecular and Cellular Biology·L M KarnitzR T Abraham
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·M C FriedmannW J Leonard
Jun 1, 1996·Current Biology : CB·L A Winston, T Hunter
Jun 1, 1996·Current Opinion in Immunology·R T Abraham
Feb 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J N Ihle
Jan 15, 1997·Genes & Development·X LiuL Hennighausen

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Citations

Jul 10, 1998·Veterinary Immunology and Immunopathology·R A KirkenW L Farrar
Sep 21, 2000·Molecular and Cellular Endocrinology·H Dinerstein-CaliM Postel-Vinay
Oct 18, 2001·Molecular and Cellular Endocrinology·H YamashitaH Rui
Oct 18, 2001·Molecular and Cellular Endocrinology·C Beisenherz-HussB Groner
Apr 12, 2003·Cellular Signalling·Michelle M FungKathleen L McGuire
Apr 1, 2000·Cytokine & Growth Factor Reviews·P M GrimleyH Rui
Nov 20, 2009·The Journal of Biological Chemistry·Jeremy A RossRobert A Kirken
Jan 24, 2013·The Journal of Biological Chemistry·Georgialina RodriguezRobert A Kirken
Jan 17, 2003·Molecular Biology of the Cell·Salvador Aznar BenitahJuan Carlos Lacal
Jun 13, 2000·Oncogene·T Decker, P Kovarik
Jun 1, 1999·FEBS Letters·S ValgeirsdóttirC H Heldin
Aug 19, 2018·The Biochemical Journal·Blanca E Ruiz-MedinaRobert A Kirken
Jan 19, 2000·Leukemia·J A McCubreyA Mufson
Dec 19, 2007·The Journal of Biological Chemistry·Jeremy A RossRobert A Kirken
Nov 6, 2001·The Journal of Biological Chemistry·Anthony D Cristillo, Barbara E Bierer
Jan 13, 2001·The Journal of Biological Chemistry·P M GowriM Vore
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M C BoscoI Espinoza-Delgado
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·F BehbodR A Kirken
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R A KirkenW L Farrar
Aug 7, 2003·Biochemical and Biophysical Research Communications·Shahab UddinLeonidas C Platanias

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