Two distinct cell sources of H2O2 in the lignifying Zinnia elegans cell culture system

Protoplasma
Laura V Gómez RosA Ros Barceló

Abstract

The use of transdifferentiating Zinnia elegans mesophyll cells has proved useful in investigations of the process of xylem differentiation from cambial derivatives. Cultured mesophyll cells can be induced by external stimuli to proceed through temporally controlled developmental programs which conclude in the formation of single-cell-derived dead vascular tracheids and parenchyma-like elements. However, there is a gap in our knowledge concerning the role played by reactive oxygen species (O(2) (-) and H(2)O(2)) in the development of these vascular elements. In this study, we show by the following four independent and highly selective methods that transdifferentiating Z. elegans mesophyll cells are capable of producing reactive oxygen species: the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay, which monitors O(2) (-) production, and the xylenol orange, 2,7-dichlorofluorescein diacetate, and CeCl(3) assays, which monitor H(2)O(2) production and localization. The joint use of these biochemical (XTT and xylenol orange) assays and cytochemical (2,7-dichlorofluorescein diacetate and CeCl(3)) probes revealed that transdifferentiating Z. elegans mesophyll cells do not show an oxidative burst but li...Continue Reading

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Citations

Mar 7, 2009·Journal of Experimental Botany·Kaisa MarjamaaKurt V Fagerstedt
Jan 4, 2012·Journal of Experimental Botany·Benjamin BollhönerHannele Tuominen
Apr 11, 2013·The Plant Cell·Edouard PesquetHannele Tuominen
May 6, 2014·Plant Physiology and Biochemistry : PPB·Joaquín HerreroJosé Miguel Zapata
Apr 3, 2013·Plant Physiology and Biochemistry : PPB·Joaquín HerreroJosé Miguel Zapata
Dec 5, 2012·Plant Physiology and Biochemistry : PPB·Sarai Belchí-NavarroMaria A Pedreño
Nov 10, 2006·The Plant Journal : for Cell and Molecular Biology·Maria Concetta de PintoLaura De Gara
Oct 8, 2008·Microscopy Research and Technique·Shaoliang ChenAndrea Polle
Apr 10, 2010·Journal of Integrative Plant Biology·Anna Kärkönen, Sanna Koutaniemi
Aug 21, 2013·Journal of Experimental Botany·Esther Novo-UzalMaría Ángeles Pedreño
Dec 3, 2014·Phytochemistry·Anna Kärkönen, Kazuyuki Kuchitsu
Apr 17, 2015·Annals of Botany·Jaime BarrosEdouard Pesquet

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