PMID: 9662032Jul 14, 1998Paper

Two distinct groups of mucin-like genes are differentially expressed in the developmental stages of Trypanosoma cruzi

Molecular and Biochemical Parasitology
L H Freitas-JuniorS Schenkman

Abstract

Sialic acid acceptors of Trypanosoma cruzi are abundant mucin-like glycoproteins linked to the parasite membrane by a glycosylphosphatidyl inositol (GPI) anchor. They are heterogeneous and variable in different parasite stages. The protein portion of these mucins contains many threonine residues, and is thought to be encoded by a heterogeneous gene family. To investigate whether the high degree of heterogeneity in the mucin gene family is responsible for the diversity of mucins expressed on the parasite surface, we have studied the expression of mucin genes in several developmental stages of T. cruzi. We have found that mucins are expressed in all parasite stages. By using conserved sequences at 3' end of translated sequences of the gene family and the splice leader sequence, we have isolated 120 mucin-like cDNAs by RT-PCR from epimastigote and trypomastigote mRNAs. All transcribed genes contain conserved 5' and 3' regions, which code for the signal peptide, the sequence for GPI anchor addition, and a conserved domain rich in threonine residues. The internal portions of these genes are highly variable in size and sequence, and can be grouped in two major categories. One group contains KP(1-2)T(6-8) repeats, a motif found in mam...Continue Reading

References

Feb 1, 1991·Experimental Parasitology·S R UlianaL M Floeter-Winter
Jan 1, 1990·Methods in Enzymology·J Hein
Jun 1, 1985·Molecular and Biochemical Parasitology·J O PreviatoL Mendonça-Previato
Oct 13, 1995·The Journal of Biological Chemistry·J M Di NoiaA C Frasch
Apr 1, 1995·Molecular and Biochemical Parasitology·M K Hughes, A L Hughes
Mar 1, 1995·Molecular and Biochemical Parasitology·M R BrionesS Schenkman
Jan 1, 1994·Annual Review of Microbiology·S SchenkmanV Nussenzweig
May 1, 1996·Molecular and Biochemical Parasitology·C M EgimaS Schenkman
Dec 13, 1996·The Journal of Biological Chemistry·J M Di NoiaA C Frasch
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Apr 21, 2001·Experimental Parasitology·C L Allen, J M Kelly
May 30, 2001·Molecular and Biochemical Parasitology·A Acosta-SerranoS Schenkman
Nov 7, 2000·Parasitology Today·S J HicksA P Corfield
Feb 21, 2006·Nature Reviews. Microbiology·Carlos A BuscagliaJavier M Di Noia
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Fernando VillaltaMaria F Lima
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·W Colli, M J Alves
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·I C AlmeidaL R Travassos
Jun 14, 2000·The Journal of Biological Chemistry·G D PollevickA C Frasch

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