PMID: 8599887Jan 1, 1996Paper

Two distinct mechanisms of cytotoxicity mediated by herpes simplex virus-specific CD4+ human cytotoxic T cell clones

Clinical Immunology and Immunopathology
Masaki YasukawaS Fujita

Abstract

The present study was undertaken to elucidate the mechanisms responsible for the cytotoxicity of herpes simplex virus (HSV)-specific CD4+ human cytotoxic T lymphocyte (CTL) clones, focusing on perforin and membrane-bound lymphotoxin (LT) (tumor necrosis factor-beta). Two HSV-specific CD4+ CTL clones, which expressed both perforin and membrane-bound LT, exerted HSV-specific cytotoxicity and cytotoxicity against LT-sensitive L929 cells. These CD4+ CTL clones lysed HSV-infected cells directly in an HLA class II-restricted manner and did not exhibit "bystander killing." The culture supernatants of these clones stimulated with HSV antigen showed no cytotoxicity against HSV-infected cells or L929 cells, suggesting that adhesion to target cells is essential to their antigen-specific and antigen-nonspecific cytotoxicities. The cytotoxicities of these clones against HSV-infected autologous cells were inhibited by an anti-CD3 monoclonal antibody but not by an anti-LT antibody. Conversely, their cytotoxicities against L929 cells appeared to be partially inhibited by the anti-LT antibody but not by the anti-CD3 monoclonal antibody. Furthermore, target cell DNA fragmentation induced by these CD4+ CTL clones was apparently observed in L929 c...Continue Reading

Citations

Apr 10, 2004·Molecular Immunology·Andreas AmbachHarald Gollnick
Feb 10, 2006·Journal of Photochemistry and Photobiology. B, Biology·Andreas AmbachHarald Gollnick
Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emmanuelle Le RoyJean-Luc Davignon
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Channakeshava Sokke UmeshappaJim Xiang
Aug 19, 2007·The Journal of General Virology·Sandra DelmasJean-Luc Davignon

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