PMID: 9419350Feb 21, 1998Paper

Two distinct populations of synaptic-like vesicles from rat brain

Proceedings of the National Academy of Sciences of the United States of America
G ThoidisK V Kandror

Abstract

In nonneuronal cells, several plasma membrane proteins such as exofacial enzymes, receptors, and ion channels recycle between their intracellular compartment(s) and the cell surface via an endosomal pathway. In neurons, however, this pathway has not been extensively characterized. In particular, it remains unclear whether or not it is related to the recycling of small synaptic vesicles, the major pathway of membrane traffic in nerve terminals. To approach this problem, we purified and studied a vesicular fraction from rat brain synaptosomes. Two distinct populations of vesicles with different buoyant densities and sedimentation coefficients were detected in this fraction by sucrose gradient centrifugation and Western blot analysis of the individual proteins. Both populations contain proteins that are markers of synaptic vesicles, namely, SV2, synaptotagmin, synaptophysin, secretory carrier membrane proteins (SCAMPs), synaptobrevin, and rab3a. A striking difference between the two populations is the presence of arginine aminopeptidase activity (a previously suggested marker for the regulated endosomal recycling pathway) exclusively in the lighter less-dense vesicles. The same two vesicular populations were also detected in the p...Continue Reading

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Citations

Jan 17, 2002·Neurochemistry International·S BiegerD Maysinger
Oct 3, 2002·Journal of Neurochemistry·Ian C G WeaverMichael J Meaney
May 16, 2000·The Journal of Physiology·K M BuckleyM T Waring
Jan 5, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E M Koehler-StecI A Simpson
Nov 1, 2006·Progress in Neurobiology·Dario BonanomiFlavia Valtorta
Feb 7, 2009·Methods in Cell Biology·Bhanu P Jena
May 13, 1999·The Journal of Biological Chemistry·G ThoidisK V Kandror
May 30, 2008·Traffic·Hao FeiDavid E Krantz
Mar 4, 2000·The Journal of Biological Chemistry·C J ProvodaK M Buckley
Sep 23, 2000·American Journal of Physiology. Endocrinology and Metabolism·C RohK V Kandror

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