Two domains of the AL1 protein mediate geminivirus origin recognition

Virology
H J GladfelterL Hanley-Bowdoin

Abstract

The geminiviruses tomato golden mosaic virus (TGMV) and bean golden mosaic virus (BGMV) have bipartite genomes. Their A and B DNA components contain cis-acting sequences that function as origins of replication, while their A components encode the trans-acting replication proteins--AL1 and AL3. Earlier experiments demonstrated that virus-specific interactions between the cis- and trans-acting functions are required for TGMV and BGMV replication and that the AL1 proteins of the two viruses specifically bind their respective origins. In the current study, characterization of AL1 and AL3 proteins produced from plant expression cassettes in transient replication assays revealed that interaction between AL1 and the origin is responsible for virus-specific replication. The AL3 protein does not contribute to specificity but can be preferred by its cognate AL1 protein when replication is impaired. Analysis of chimeric proteins showed that two regions of AL1 act as specificity determinants during replication. The first domain is located between amino acids 1 and 116 and recognizes the AL1 origin binding site. The second region, which is between amino acids 121 and 209, is not dependent on the known AL1 DNA binding site. Analysis of wild ...Continue Reading

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Citations

Jan 13, 2004·Molecular Plant-microbe Interactions : MPMI·Luke A SelthM Ali Rezaian
Nov 23, 2000·The Plant Cell·M R Morra, I T Petty
Nov 19, 2010·Journal of Virology·Tara E NashLinda Hanley-Bowdoin
Apr 10, 2010·BMC Evolutionary Biology·Rob W BriddonClaude M Fauquet
Mar 26, 1998·Virology·B M OrozcoL Hanley-Bowdoin
Apr 24, 2008·Virology·Gabriela Lacatus, Garry Sunter
Mar 26, 2010·The Journal of General Virology·Basavaprabhu L Patil, Claude M Fauquet
Sep 6, 2019·Archives of Virology·Jennifer Guerrero, Garry Sunter
May 9, 2007·The Journal of General Virology·Janet A WillmentEdward P Rybicki

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