Two dominant selectable markers for genetic manipulation in Neurospora crassa.

Current Genetics
Lingaonan HeQun He

Abstract

Neurospora crassa is an excellent model fungus for studies on molecular genetics, biochemistry, physiology, and molecular cell biology. Along with the rapid progress of Neurospora research, new tools facilitating more efficient and accurate genetic analysis are in high demand. Here, we tested whether the dominant selective makers widely used in yeasts are applicable in N. crassa. Among them, we found that the strains of N. crassa are sensitive to the aminoglycoside antibiotics, G418 and nourseothricin. 1000 μg/mL of G418 or 50 μg/mL of nourseothricin is sufficient to inhibit Neurospora growth completely. When the neomycin phosphotransferase gene (neo) used in mammalian cells is expressed, N. crassa shows potent resistance to G418. This establishes G418-resistant marker as a dominant selectable marker to use in N. crassa. Similarly, when the nourseothricin acetyltransferase gene (nat) from Streptomyces noursei is induced by qa-2 promoter in the presence of quinic acid (QA), N. crassa shows potent resistance to nourseothricin. When nat is constitutively expressed by full-length or truncated versions of the promoter from the N. crassa cfp gene (NCU02193), or by the trpC promoter of Aspergillus nidulans, the growth of N. crassa in ...Continue Reading

References

Jul 25, 1981·Journal of Molecular Biology·F Colbère-GarapinA C Garapin
Apr 18, 2002·Genes & Development·Yuhong YangYi Liu
May 4, 2002·Nature Reviews. Genetics·Rowland H Davis, David D Perkins
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Katherine A BorkovichRobert Pratt
Aug 18, 2004·Trends in Genetics : TIG·James E Galagan, Eric U Selker
Oct 12, 2004·Gene·Oliver ReussJoachim Morschhäuser
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hildur V ColotJay C Dunlap
Oct 27, 2009·Biochemical and Biophysical Research Communications·Min Su KimYong-Sun Bahn
Dec 2, 2009·The Journal of Biological Chemistry·Yuanbiao ZhaoQun He
Jun 24, 2015·Antimicrobial Agents and Chemotherapy·Kangji WangShaojie Li

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Citations

Aug 1, 2020·Frontiers in Bioengineering and Biotechnology·Teng ZhangChun Li

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Methods Mentioned

BETA
phosphotransferase
RIP
PCR
gene knockout

Related Concepts

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Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

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