PMID: 6405041Feb 5, 1983Paper

Two Drosophila actin genes in detail. Gene structure, protein structure and transcription during development

Journal of Molecular Biology
F SánchezB J McCarthy

Abstract

DNA fragments representing the six Drosophila actin genes have been isolated by recombinant DNA techniques. We have compared the transcriptional characteristics of the actin genes at the cytological loci 79B and 88F. The activity of each gene in vivo was examined using gene-specific probes from transcribed, but non-translated 3' regions of each gene. The genes show similar patterns of transcriptional activity during development until the pupal stage, with two periods showing RNA accumulation at two to three hours and 12 to 15 hours during embryonic development, followed by large increases in the proportion of message from each gene in first and second instar larvae. During pupal development, the 88F gene apparently produces a larger proportion of transcripts than at any other developmental stage, while the transcripts of the 79B gene are reduced to a level lower than in first and second instar larvae. The 5' end of each messenger RNA in larvae has been mapped by nuclease S1 digestion of hybrids between restriction fragments of genes and homologous mRNAs. The two genes display widely differing capacities to serve as templates for transcription in vitro in HeLa cell extracts. The complete DNA sequences of both genes including the...Continue Reading

References

Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Vandekerckhove, K Weber
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M ElzingaR S Adelstein
Nov 1, 1970·In Vitro·G Echalier, A Ohanessian
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N ArnheimE Zimmer
Jan 1, 1951·Cold Spring Harbor Symposia on Quantitative Biology·E B LEWIS

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Citations

Feb 1, 1990·Plant Molecular Biology·D McElroyR Wu
May 1, 1992·Journal of Molecular Evolution·N MounierJ C Prudhomme
Nov 1, 1990·Current Genetics·B CresnarR Schmitt
Jan 1, 1988·Journal of Molecular Evolution·G S CrossH R Woodland
Jan 1, 1985·Journal of Molecular Evolution·D R ForanG P Moore
Oct 1, 1983·Developmental Biology·E A CraigL J Manseau
Jun 1, 1989·Developmental Biology·C L Courchesne-Smith, S L Tobin
Sep 15, 1984·Journal of Molecular Biology·M P Vitek, E M Berger
Dec 1, 1985·Molecular and Biochemical Parasitology·A H DavisP Klich
Jul 1, 1987·Molecular and Biochemical Parasitology·M HuberS Rozenblatt
Jan 15, 1988·Molecular and Biochemical Parasitology·J G WesselingJ G Schoenmakers
Aug 1, 1988·Molecular and Biochemical Parasitology·J G WesselingJ G Schoenmakers
Jun 15, 1989·Molecular and Biochemical Parasitology·J G WesselingJ G Schoenmakers
Jun 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N Mounier, J C Sparrow
Oct 1, 1994·Insect Biochemistry and Molecular Biology·M He, D S Haymer
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K MogamiC P Emerson
Nov 1, 1985·The Journal of Cell Biology·J A Winkles, R M Grainger
Jul 11, 1985·Nucleic Acids Research·E B Keller, W A Noon
Apr 24, 1987·Nucleic Acids Research·M K YamanakaS L Tobin
Sep 11, 1990·Nucleic Acids Research·M T Macias, L Sastre
Jan 1, 1992·Insect Molecular Biology·M He, D S Haymer
Jun 20, 1998·Molecular Ecology·F X VillablancaS R Palumbi
Jun 20, 1986·Journal of Molecular Biology·L CherbasP Cherbas
Jul 20, 1985·Journal of Molecular Biology·P F MartinW Sofer
Jan 1, 1997·DNA Sequence : the Journal of DNA Sequencing and Mapping·V LardansC Dissous

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