Two effective methods for correcting experimental high-throughput screening data

Bioinformatics
Plamen DragievVladimir Makarenkov

Abstract

Rapid advances in biomedical sciences and genetics have increased the pressure on drug development companies to promptly translate new knowledge into treatments for disease. Impelled by the demand and facilitated by technological progress, the number of compounds evaluated during the initial high-throughput screening (HTS) step of drug discovery process has steadily increased. As a highly automated large-scale process, HTS is prone to systematic error caused by various technological and environmental factors. A number of error correction methods have been designed to reduce the effect of systematic error in experimental HTS (Brideau et al., 2003; Carralot et al., 2012; Kevorkov and Makarenkov, 2005; Makarenkov et al., 2007; Malo et al., 2010). Despite their power to correct systematic error when it is present, the applicability of those methods in practice is limited by the fact that they can potentially introduce a bias when applied to unbiased data. We describe two new methods for eliminating systematic error from HTS data based on a prior knowledge of the error location. This information can be obtained using a specific version of the t-test or of the χ(2) goodness-of-fit test as discussed in Dragiev et al. (2011). We will s...Continue Reading

References

Jun 6, 2000·Journal of Biomolecular Screening·J H ZhangK R Oldenburg
Jan 9, 2004·Journal of Biomolecular Screening·Christine BrideauAndy Liaw
Aug 17, 2005·Journal of Biomolecular Screening·Dmytro Kevorkov, Vladimir Makarenkov
Oct 14, 2005·Journal of the American Chemical Society·Jeremiah S HelmSuzanne Walker
Feb 9, 2006·Nature Biotechnology·Nathalie MaloRobert Nadon
Jun 24, 2008·Journal of Biomolecular Screening·Xiaohua Douglas Zhang
Sep 8, 2010·Journal of Biomolecular Screening·Nathalie MaloRobert Nadon
Jan 21, 2011·BMC Bioinformatics·Plamen DragievVladimir Makarenkov
Nov 29, 2011·Bioinformatics·Jean-Philippe CarralotThierry Dorval

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Citations

May 16, 2014·Journal of Biomolecular Screening·Chand S MangatEric D Brown
Oct 30, 2016·Briefings in Bioinformatics·Shardul ParicharakGerard J P van Westen

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