Two ELISAs to detect anti-neutrophil cytoplasm antibodies (ANCA) in various vasculitides.

Pathology
J A SavigeD J Davies

Abstract

Anti-neutrophil cytoplasm antibodies (ANCA) are present in the serum of patients with Wegener's granulomatosis, microscopic polyarteritis, and some other small vessel vasculitides. There are at least 2 different anti-neutrophil cytoplasm antibodies identified by their distinctive cytoplasmic staining patterns on indirect immunofluorescence examination. The only antigen identified to date is myeloperoxidase which has a perinuclear distribution on alcohol-fixed neutrophils and monocytes. We have established an ELISA that detects all anti-neutrophil cytoplasm antibodies and one specific for anti-myeloperoxidase antibodies. In the ELISA for anti-neutrophil cytoplasm antibodies, all sera with diffuse cytoplasmic or perinuclear neutrophil staining on indirect immunofluorescence examination bound at levels greater than the normal range (34%, m + 4SD). Three convalescent sera that were negative by indirect immunofluorescence examination were also negative in the assay. Positive sera could be detected at a dilution of 2 and inhibition studies showed that the binding was specific for the neutrophil extract. However, the presence of anti-neutrophil antibodies (ANA), anti-mitochondrial antibodies or immune complexes resulted occasionally i...Continue Reading

Citations

Feb 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M C NashH R Powell
Aug 1, 1991·Australian and New Zealand Journal of Medicine·J A SavigeJ D Parkin
Jun 1, 1994·Australian and New Zealand Journal of Medicine·J A SavigeJ C Duggan
Mar 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·E SoppiP Pelkonen
Aug 1, 1991·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J Wieslander
Dec 20, 2007·American Journal of Clinical Pathology·Michelle TrevisinJudy Savige
Jan 1, 1994·Acta Oto-laryngologica. Supplementum·K InamuraY Koike
Feb 1, 1994·Clinical and Experimental Immunology·J G JenningsJ A Savige
Apr 21, 2009·Anais Brasileiros De Dermatologia·Hebert Roberto Clivati BrandtPaulo Ricardo Criado

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