Two female-specific DSX proteins are encoded by the sex-specific transcripts of dsx, and are required for female sexual differentiation in two wild silkmoth species, Antheraea assama and Antheraea mylitta (Lepidoptera, Saturniidae)

Insect Biochemistry and Molecular Biology
Jayendra Nath Shukla, J Nagaraju

Abstract

doublesex (dsx) is the bottom most gene of the sex-determination cascade of Drosophila melanogaster. The pre-mRNA of dsx splices to produce male- and female-specific transcripts which code for the male- and female-specific proteins, respectively. dsx homologues have been characterized from different (many in Diptera, two in Hypmenoptera and only one in Lepidoptera) insect species. Sex-specific splice forms of dsx pre-mRNA in all these species code for one male- and one female-specific DSX proteins, which regulate the downstream target genes responsible for sex-specific characters. In the present study we have cloned and characterized the dsx homologues from two saturniid silkmoths, Antheraea assama and Antheraea mylitta. The divergence time between Saturniidae and Bombycidae to which the domesticated silkworm, Bombyx mori belongs is estimated to be around 160.9 MY. Interestingly, the dsx pre-mRNA of these wild silkmoths sex-specifically splices to generate multiple splice variants. On the basis of their open reading frame (ORF) and conceptual translation, two female-specific (DSX(F1) and DSX(F2)) and one male-specific (DSX(M)) proteins could be inferred, in both the moths. Presence or absence of a 15 bp stretch within the ORF o...Continue Reading

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Citations

Aug 18, 2010·Genetica·Jayendra Nath ShuklaJavaregowda Nagaraju
Nov 19, 2013·Insect Biochemistry and Molecular Biology·Xiu-Ye WangYong-Zhen Xu
Oct 30, 2012·Genetics·Edupalli V SubbaiahJavaregowda Nagaraju
Sep 30, 2010·Journal of Genetics·Tarig Dafa'allaLuke Alphey
Sep 30, 2010·Journal of Genetics·J N Shukla, J Nagaraju
Aug 24, 2010·Insect Biochemistry and Molecular Biology·Takafumi N SugimotoYukio Ishikawa
Dec 12, 2012·Scientific Reports·Jayendra Nath Shukla, Subba Reddy Palli
Mar 16, 2016·Transgenic Research·Jianping DuanQingyou Xia
Mar 24, 2015·Briefings in Functional Genomics·Eveline C Verhulst, Louis van de Zande
Dec 25, 2012·Biochemical and Biophysical Research Communications·Jianping DuanQingyou Xia
Nov 22, 2016·Insect Biochemistry and Molecular Biology·Jun XuYongping Huang
May 1, 2021·Animals : an Open Access Journal From MDPI·Chhavi ChoudharyJayendra Nath Shukla

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