PMID: 6985267Oct 1, 1982Paper

Two forms of murine epidermal growth factor: rapid separation by using reverse-phase HPLC

Proceedings of the National Academy of Sciences of the United States of America
A W BurgessE C Nice

Abstract

Epidermal growth factor (EGF) has been isolated from acid extracts of C57BL6/J mouse submaxillary glands by using hydrophobic chromatography. High yields of EGF in large amounts (10 mg) can be isolated reliably from the acid extract of the glands in less than 4 hr. The reverse-phase HPLC techniques used to purify the EGF initially yielded what appeared to be a single homogeneous EGF molecule. However, ion pairing reagents (e.g., heptafluorobutyric acid) altered the chromatographic properties, revealing two distinct species: EGF-alpha and EGF-beta. The apparent molecular weights, isoelectric points, and antigenic properties of EGF-alpha and EGF-beta were identical, and both forms stimulated a mitogenic response in 3T3 cells. Analysis of different preparations of purified EGF (commercial and experimental) indicated the presence of EGF-alpha and EGF-beta in constant proportion. Previous EGF binding studies must have used mixtures of 125I-labeled EGF-alpha and 125I-labeled EGF-beta. The two molecules appear to compete for an identical receptor on the cell surface.

References

Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
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Mar 1, 1981·The American Journal of Anatomy·M Tom-Moy, T Barka

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Citations

Aug 15, 1985·Biochemical and Biophysical Research Communications·K J HyverC Fenselau
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·E C Nice, R J Simpson
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G S BaldwinR J Simpson
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J M BestermanP Cuatrecasas
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G T MontelioneH A Scheraga
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G T MontelioneH A Scheraga
Oct 11, 1988·Nucleic Acids Research·D S Dorow, R J Simpson
Apr 30, 2003·Biomedical Chromatography : BMC·Laura ZamursEdouard Nice
Apr 29, 1987·Biochemical and Biophysical Research Communications·K StrombergD N Orth
Jan 1, 1985·Differentiation; Research in Biological Diversity·K W Pyke, R L Gogerly
Sep 1, 1987·Analytical Biochemistry·R P DiAugustineM Walker
Jul 12, 1988·Biochemistry·A W BurgessE C Nice
Jan 26, 1988·Biochemistry·C George-NascimentoA Randolph
Aug 1, 1985·Journal of Cellular Physiology·H T HaiglerT J Moehring
Apr 22, 2005·The Journal of Biological Chemistry·Marguerite S BuzzaPhillip I Bird
Nov 15, 1990·International Journal of Cancer. Journal International Du Cancer·R H WhitheadA W Burgess
Jan 1, 1984·Journal of Cellular Biochemistry·J H KochB A Moss
Mar 2, 1984·Journal of Chromatography·A McLeod, S P Wood

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