Two functionally distinct CYP4G genes of Anopheles gambiae contribute to cuticular hydrocarbon biosynthesis.

Insect Biochemistry and Molecular Biology
Mary KefiJohn Vontas

Abstract

Cuticular hydrocarbon (CHC) biosynthesis is a major pathway of insect physiology. In Drosophila melanogaster the cytochrome P450 CYP4G1 catalyses the insect-specific oxidative decarbonylation step, while in the malaria vector Anopheles gambiae, two CYP4G paralogues, CYP4G16 and CYP4G17 are present. Analysis of the subcellular localization of CYP4G17 and CYP4G16 in larval and pupal stages revealed that CYP4G16 preserves its PM localization across developmental stages analyzed; however CYPG17 is differentially localized in two distinct types of pupal oenocytes, presumably oenocytes of larval and adult developmental specificity. Western blot analysis showed the presence of two CYP4G17 forms, potentially associated with each oenocyte type. Both An. gambiae CYP4Gs were expressed in D. melanogaster flies in a Cyp4g1 silenced background in order to functionally characterize them in vivo. CYP4G16, CYP4G17 or their combination rescued the lethal phenotype of Cyp4g1-knock down flies, demonstrating that CYP4G17 is also a functional decarbonylase, albeit of somewhat lower efficiency than CYP4G16 in Drosophila. Flies expressing mosquito CYP4G16 and/or CYP4G17 produced similar CHC profiles to 'wild-type' flies expressing the endogenous CYP4G...Continue Reading

Citations

Jan 9, 2021·Annual Review of Entomology·Gary J Blomquist, Matthew D Ginzel
Aug 20, 2019·Insect Biochemistry and Molecular Biology·Lixian WuSheng Li
Aug 7, 2021·Insects·Fiza ArshadMonika Gulia-Nuss
Sep 2, 2021·Insect Biochemistry and Molecular Biology·Dries AmezianGaëlle Le Goff
Oct 1, 2021·Annual Review of Entomology·Ralf NauenJohn Vontas
Mar 20, 2022·Journal of Insect Physiology·Zhe-Chao WangYan-Yuan Bao

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