Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation

European Journal of Biochemistry
Greco HernándezR Rivera-Pomar

Abstract

Eukaryotic initiation factor (eIF) 4B is part of the protein complex involved in the recognition and binding of mRNA to the ribosome. DrosophilaeIF4B is a single-copy gene that encodes two isoforms, termed eIF4B-L (52.2 kDa) and eIF4B-S (44.2 kDa), generated as a result of the alternative recognition of two polyadeynlation signals during transcription termination and subsequent alternative splicing of the two pre-mRNAs. Both eIF4B mRNAs and proteins are expressed during the entire embryogenesis and life cycle. The proteins are cytoplasmic with polarized distribution. The two isoforms bind RNA with the same affinity. eIF4B-L and eIF4B-S preferentially enhance cap-dependent over IRES-dependent translation initiation in a Drosophila cell-free translation system. RNA interference experiments suggest that eIF4B is required for cell survival, although only a modest reduction in rate of protein synthesis is observed. Overexpression of eIF4B in Drosophila cells in culture and in developing eye imaginal discs promotes cell proliferation.

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R Benne, J W Hershey
Mar 1, 1990·Molecular and Cellular Biology·F RozenN Sonenberg
May 1, 1989·Molecular and Cellular Biology·F G Maroto, J M Sierra
Oct 22, 1982·Science·G M Rubin, A C Spradling
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Apr 26, 1995·Biochimica Et Biophysica Acta·G Hernández, J M Sierra
Sep 1, 1993·Mechanisms of Development·M Klingler, J P Gergen
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·J de la CruzP Linder
Apr 29, 1998·The Journal of Biological Chemistry·N J Richter-CookW C Merrick
Dec 22, 1999·Biochemical and Biophysical Research Communications·A M MetzK S Browning
Jan 5, 2000·Genes & Development·T TuschlP A Sharp
Mar 25, 2000·Science·M D AdamsJ C Venter
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·J C ClemensJ E Dixon
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
Oct 6, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·M KurisawaT Okano
May 1, 1997·Development Genes and Evolution·Britt WildemannG Bicker

❮ Previous
Next ❯

Citations

Jan 21, 2010·Molecular and Cellular Biology·David ShahbazianNahum Sonenberg
Feb 26, 2010·BMC Research Notes·Alison E BarnhillHeather West Greenlee
Apr 14, 2010·Ageing Research Reviews·Daniel S EvansLutz Kockel
Jun 6, 2012·Comparative and Functional Genomics·Greco HernándezArmen Parsyan
Apr 5, 2005·Mechanisms of Development·Greco HernándezRolando Rivera-Pomar
Jun 1, 2005·Mechanisms of Development·Greco Hernández, Paula Vazquez-Pianzola
Apr 27, 2019·Acta Neuropathologica Communications·Lindsey D GoodmanNancy M Bonini
Aug 6, 2016·Fly·Steven J MarygoldPaul Lasko
Sep 28, 2021·Frontiers in Immunology·Biao ChenJi-Long Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.