Two genes, COL4A3 and COL4A4 coding for the human alpha3(IV) and alpha4(IV) collagen chains are arranged head-to-head on chromosome 2q36

FEBS Letters
R MomotaY Ninomiya

Abstract

We first isolated and characterized genomic DNA fragments that cover the 5' flanking sequences of COL4A3 and COL4A4 encoding the human basement membrane alpha3(IV) and alpha4(IV) collagen chains, respectively. Nucleotide sequence analysis indicated that the two genes are arranged head-to-head. To determine transcription start site for COL4A4 gene, we performed RACE and RNase protection assays, indicating that there are two alternative transcripts presumably derived from two different promoters. Interestingly, one transcription start site (from exon 1') of COL4A4 is only 5 bp away from the reported transcription start site of COL4A3, whereas the other transcript (from exon 1) starts 373 nucleotides downstream from the first one, generating the two kinds of transcripts that differ in the 5' UTR regions. Expression of these two transcripts appears tissue-specific; exon 1 transcript was expressed predominantly in epithelial cells, while exon 1' transcript showed rather ubiquitous and low expression. The nucleotide sequence of the promoter region is composed of dense CpG dinucleotides, GC boxes, CTC boxes and a CCAAT box but no TATA box. These results provide information to delineate the promoter activity for the tissue-specific exp...Continue Reading

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Citations

Nov 16, 2005·Nephron. Experimental Nephrology·Catherine AlexakisGeorge Bou-Gharios
Aug 2, 2014·Ophthalmic Genetics·Nikolaos S KokolakisMarilita M Moschos
Sep 19, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yao Yao
Aug 8, 2002·Archives of Histology and Cytology·Norio UrabeYoshifumi Ninomiya
Feb 13, 2013·BMC Medical Genomics·Guohua WangYadong Wang
Jul 30, 2016·Journal of Cellular and Molecular Medicine·Yuan WuHao Deng
Aug 26, 2016·Scientific Reports·Yuan FangWenli Zhang
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Mar 25, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Cédric MeerssemanDominique Rocha
Jan 3, 2001·Journal of the American Society of Nephrology : JASN·Laurence HeidetCorinne Antignac
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Feb 28, 2009·BMC Bioinformatics·Quan WangMinghua Deng
Jan 7, 2004·Genome Research·Nathan D TrinkleinRichard M Myers

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