Two genetic changes in cis-regulatory elements caused evolution of petal spot position in Clarkia

Nature Plants
Peng Jiang, Mark D Rausher

Abstract

A major premise in evolutionary developmental biology is that regulatory changes, often involving cis-regulatory elements, are responsible for much morphological evolution. This premise is supported by recent investigations of animal development, but information is just beginning to accumulate regarding whether it also applies to the evolution of plant morphology1-4. Here, we identify the genetic differences between species in the genus Clarkia that are responsible for evolutionary change in an ecologically important element of floral colour patterns: spot position. The evolutionary shift in spot position was due to two simple genetic changes that resulted in the appearance of a transcription factor binding site mutation in the R2R3 Myb gene that changes spot formation. These genetic changes caused R2R3 Myb to be activated by a different transcription factor that is expressed in a different position in the petal. These results suggest that the regulatory rewiring paradigm is as applicable to plants as it is to animals, and support the hypothesis that cis-regulatory changes may often play a role in plant morphological evolution.

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Citations

Jan 3, 2018·Nature Plants·Róisín Fattorini, Beverley J Glover
Nov 22, 2018·American Journal of Botany·Philipp M Schlüter
Nov 18, 2018·The Plant Journal : for Cell and Molecular Biology·Maricris L ZaidemMichael D Purugganan
Apr 6, 2019·Journal of Experimental Botany·Misha PaauwFrancesca M Quattrocchio
Sep 4, 2019·Nature Plants·Hongyan ShanHongzhi Kong
Nov 30, 2019·Nature Communications·Ying BaoJonathan F Wendel
Apr 30, 2019·Frontiers in Plant Science·Mohsen HajheidariMarcel Bucher

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Methods Mentioned

BETA
1-hybrid
PCR
Y1H
transfection
one hybrid
Assay

Software Mentioned

pGL
PlantCARE
Softberry TSSP
3AT

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