Jan 1, 1976

Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties

Journal of Biochemistry
H HatanakaF Egami


Two glycosulfatases [EC], I and II, were purified 31.3- and 33.9-fold respectively, from a crude extract of the liver of Charonia lampas. The purification was carried out by the following chromatographic procedures; phosphocellulose, Sephadex G-150, Concanavalin A-Sepharose and isoelectric focussing. The enzyme preparations obtained were practically free from arylsulfatase [EC] contamination. Both glycosulfatases are probably glycoproteins differing in their carbohydrate moieties. The molecular weights of glycosulfatase I and II were estimated to be about 112,000 and 79,000 respectively. They had the same optimum pH of 5.5, and the same Km value of 25.0 mM for glucose 6-sulfate.

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Mentioned in this Paper

Sephadex G
Glucose, (beta-D)-Isomer
Enzyme agent
Enzyme preparations, digestives
Purification Aspects
concanavalin A-sepharose

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