PMID: 8955282Dec 1, 1996Paper

Two glycosyltransferase genes, lgtF and rfaK, constitute the lipooligosaccharide ice (inner core extension) biosynthesis operon of Neisseria meningitidis

Journal of Bacteriology
C M KahlerD S Stephens

Abstract

We have characterized an operon required for inner-core biosynthesis of the lipooligosaccharide (LOS) of Neisseria meningitidis. Using Tn916 mutagenesis, we recently identified the alpha-1,2-N-acetylglucosamine (GlcNAc) transferase gene (rfaK), which when inactivated prevents the addition of GlcNAc and alpha chain to the meningococcal LOS inner core (C. M. Kahler, R. W. Carlson, M. M. Rahman, L. E. Martin, and D. S. Stephens, J. Bacteriol. 178:1265-1273, 1996). During the study of rfaK, a second open reading frame (lgtF) of 720 bp was found upstream of rfaK. An amino acid sequence homology search of the GenBank and EMBL databases revealed that the amino terminus of LgtF has significant homology with a family of beta-glycosyltransferases involved in the biosynthesis of polysaccharides and O antigen of lipopolysaccharides. The chromosomal copy of lgtF was mutagenized with a nonpolar antibiotic resistance cassette to minimize potential polar effects on rfaK. Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and composition analysis of the LOS from the nonpolar lgtF mutant showed that this strain produced a truncated LOS structure which contained a LOS inner core of GlcNAc1Hep2KDO2lipid A but without the addition of...Continue Reading

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Citations

Oct 10, 1998·Molecular Microbiology·M P JenningsE R Moxon
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Mar 16, 2019·Frontiers in Immunology·Sunita GulatiPeter A Rice
Oct 20, 2018·PloS One·James Amor Smith, Maor Bar-Peled
Feb 24, 2001·Journal of Bacteriology·D ArkingD C Stein
Nov 12, 2002·Journal of Bacteriology·Andrzej Piekarowicz, Daniel C Stein
Mar 26, 2003·Infection and Immunity·Kyungcheol YiIgor Stojiljkovic
Dec 25, 2007·Journal of Bacteriology·Margaret I KanipesMario A Monteiro

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