Two grapevine metacaspase genes mediate ETI-like cell death in grapevine defence against infection of Plasmopara viticola

Protoplasma
Peijie GongPeter Nick

Abstract

Metacaspase, as hypersensitive response (HR) executors, has been identified in many plant species. Previously, the entire gene family of metacaspase has been uncovered, but there are still questions that remain unclear regarding HR-regulating gene members. In this study, based on metacaspase expression during different grapevine genotypes interacting with Plasmopara viticola, we identified MC2 and MC5 as candidates involved in HR. We overexpressed both metacaspases as GFP fusions in tobacco BY-2 cells to address subcellular localization and cellular functions. We found MC2 located at the ER, while MC5 was nucleocytoplasmic. In these overexpressor lines, cell death elicited by the bacterial protein harpin, is significantly enhanced, indicating MC2 and MC5 mediated defence-related programmed cell death (PCD). This effect was mitigated, when the membrane-located NADPH oxidase was inhibited by the specific inhibitor diphenylene iodonium, or when cells were complemented with methyl jasmonate, a crucial signal of basal immunity. Both findings are consistent with a role of MC2 and MC5 in cell death-related immunity. Using a dual-luciferase reporter system in grapevine cells we demonstrated both MC2 and MC5 promoter alleles from V. rup...Continue Reading

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Citations

Jan 25, 2020·International Journal of Molecular Sciences·Yangyang LuoDong Duan
Oct 13, 2020·Frontiers in Plant Science·Mohammed Mahmood SarheedPeter Nick
Oct 28, 2020·The Plant Journal : for Cell and Molecular Biology·Qing-Ling LuanGuan-Feng Wang
Feb 26, 2021·Journal of Experimental Botany·Rita B Santos, Andreia Figueiredo
Jun 3, 2021·Cells·Huimin RenShenkui Liu
Aug 6, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Juho HautsaloUNKNOWN PPP Barley Consortium
Dec 9, 2020·Plant Science : an International Journal of Experimental Plant Biology·Hao WangPeter Nick

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Datasets Mentioned

BETA
AF369524
KY069974
KY069975
KY069976
KY069978
KY069977
KY069979
as

Methods Mentioned

BETA
confocal microscopy
transgenic
electrophoresis
PCR
transfection

Software Mentioned

MARARS
DNAMAN
MEGA
ClustalW
BLAST
Zen

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