PMID: 7015326Feb 1, 1981Paper

Two growth factors and two hormones regulate initiation of DNA synthesis in cultured mouse cells through different pathways of events

Proceedings of the National Academy of Sciences of the United States of America
L Jimenez de AsuaA M Otto

Abstract

Epidermal growth factor (EGF) as well as prostaglandin F2 alpha (PGF2 alpha), when added to quiescent, confluent Swiss 3T3 cells, stimulate the initiation of DNA synthesis, which occurs with apparent first-order kinetics after a lag phase of 14-15 hr. These two growth factors appear to stimulate similar events; insulin enhances and hydrocortisone can inhibit the stimulatory effect of either. Here we show that the addition of EGF and PGF2 alpha together, however, results in a synergistic effect seen at the end of the lag phase, but only when EGF and PGF2 alpha are added within 6 hr of each other. Addition of one growth factor 10 or 15 hr after the other delayed the synergy for 15 hr after the addition of the second growth factor. Insulin further increased the rate of entry into the s phage stimulated by EGF and PGF2 alpha together, whereas hydrocortisone inhibited the stimulatory effect observed with either EGF or PGF2 alpha alone. These results suggest that, in spite of the common events responsible for the interactions with the two hormones, EGF and PGF2 alpha must have differences in their sequences of events that initiate DNA synthesis.

References

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Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·V G RiddleA B Pardee
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A M OttoL Jimenez de Asua
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Citations

Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A R OlivierG Thomas
Aug 1, 1987·The Journal of Cell Biology·C H Clegg, S D Hauschka
Mar 1, 1988·The Journal of Cell Biology·Y C Wang, P A Rubenstein
May 1, 1982·The Journal of Cell Biology·P Sherline, R N Mascardo
Oct 1, 1991·Pharmacology & Therapeutics·E K Rowinsky, R C Donehower
Apr 1, 1985·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·R van WijkL Jimenez de Asua
Jul 1, 1988·Journal of Cellular Physiology·R M Böhmer, L D Beattie
Apr 1, 1982·Experimental Cell Research·B J OrtwerthC Conlon-Hollingshead
Feb 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A Lupulescu
Nov 1, 1985·Journal of Clinical Pharmacology·N CharletR J Wills
Sep 1, 1982·Journal of Cellular Physiology·W K Miskimins, N Shimizu
May 1, 1984·Journal of Cellular Physiology·R van WijkL Jimenez de Asua
Jan 1, 1986·Journal of Cellular Physiology·R Katoh-SembaS Varon
Dec 1, 1984·Journal of Cellular Physiology·K M RichmondR F Brooks
Jul 30, 1997·Biochemical and Biophysical Research Communications·C S LevyL Jimenez de Asua
Sep 1, 1984·Molecular and Cellular Biology·J Campisi, A B Pardee

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