Two homologous apolipoprotein AI mimetic peptides. Relationship between membrane interactions and biological activity

The Journal of Biological Chemistry
R F EpandG M Anantharamaiah

Abstract

Two related 18-amino acid, class A, amphipathic helical peptides termed 3F-2 and 3F14 were chosen for this study. Although they have identical amino acid compositions and many similar biophysical properties, 3F-2 is more potent than 3F14 as an apolipoprotein AI mimetic peptide. The two peptides exhibit similar gross conformational properties, forming structures of high helical content on a membrane surface. However, the thermal denaturation transition of 3F-2 is more cooperative, suggesting a higher degree of oligomerization on the membrane. Both 3F-2 and 3F14 promote the segregation of cholesterol in membranes containing phosphatidylcholine and cholesterol, but 3F-2 exhibits a greater selectivity for partitioning into cholesterol-depleted regions of the membrane. Magic angle spinning/NMR studies indicate that the aromatic residues of 3F-2 are stacked in the presence of lipid. The aromatic side chains of this peptide also penetrate more deeply into membranes of phosphatidylcholine with cholesterol compared with 3F14. Using the fluorescent probe, 1,3-dipyrenylpropane, we monitored the properties of the lipid hydrocarbon environment. 3F-2 had a greater effect in altering the properties of the hydrocarbon region of the membrane. T...Continue Reading

References

Dec 10, 2003·Biochemistry·Richard M EpandRaquel F Epand
Apr 13, 2004·The Journal of Biological Chemistry·Geeta DattaG M Anantharamaiah
Apr 28, 2004·Biochemistry·Richard M EpandG M Anantharamaiah
Nov 3, 2004·Biochimica Et Biophysica Acta·Richard M Epand

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Citations

May 9, 2006·Current Opinion in Lipidology·Gm AnantharamaiahAlan M Fogelman
Jul 16, 2008·Journal of Lipid Research·Brian J Van LentenAlan M Fogelman
Dec 3, 2010·Journal of Clinical Lipidology·Sergio Fazio, Macrae F Linton
May 30, 2006·Biophysical Chemistry·Richard M Epand
Jun 30, 2009·Biochimica Et Biophysica Acta·Raquel F EpandRichard M Epand
Apr 16, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Mohamad NavabAlan M Fogelman
Jun 16, 2005·Current Opinion in Cardiology·C Daniel Meyers, Moti L Kashyap
Jun 9, 2006·Archives of Biochemistry and Biophysics·Su Duy NguyenDai-Eun Sok

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