Abstract
BALB/c mice were immunized with human melanoma cells and their spleen cells hybridized with NS-1 myeloma cells. The hybrids were screened for the production of antibodies that bound to melanoma cells. Two hybridomas of interesting specificity were identified and cloned. Hybridoma 5.1 produce an IgG1 antibody that binds to about half of the melanomas and carcinomas tested. The target is a polypeptide with an apparent molecular weight of 210 kilodaltons on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The antigen, denoted p210, is also expressed in normal adult brain and in certain fetal tissues. Hybridoma 6.1 produces an IgM antibody that binds to about 50% of the melanomas, and 80% of the kidney carcinomas tested. The antigen defined by this antibody in melanomas has an apparent molecular weight of 155 kilodaltons and is denoted p155. It has not been observed on any normal adult or fetal tissues. The antigen present in the kidney carcinomas was not p155, but rather consisted of two proteins of approximately 60,000 and 250,000-300,000 daltons. This observation suggests the possibility that the antigenic determinant recognized by antibody 6.1 may be present on several distinct protein molecules.
References
Jul 1, 1978·Immunochemistry·P L EyC R Jenkin
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H KoprowskiM Herlyn
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Y YehK E Hellström
Jan 1, 1979·Journal of Immunological Methods·J P BrownI Hellström
Mar 16, 1979·Science·R H Kennett, F Gilbert
Jul 1, 1979·Analytical Biochemistry·E CadmanJ Eichberg
Aug 1, 1979·European Journal of Immunology·D HerlynH Koprowski
Oct 1, 1976·The Journal of Experimental Medicine·H ShikuH F Oettgen
Dec 15, 1975·International Journal of Cancer. Journal International Du Cancer·S CornainP Rümke
Aug 7, 1975·Nature·G Köhler, C Milstein
Jan 15, 1971·International Journal of Cancer. Journal International Du Cancer·I HellströmG A Warner
Aug 15, 1974·International Journal of Cancer. Journal International Du Cancer·M A BeanH F Oettgen
Jan 1, 1980·Contemporary Topics in Immunobiology·K E HellströmI Hellström
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J P BrownK E Hellström
Feb 15, 1981·International Journal of Cancer. Journal International Du Cancer·R G WoodburyI Hellström
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R G WoodburyK E Hellström
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W G DippoldL J Old
Citations
Jan 1, 1983·Cancer Immunology, Immunotherapy : CII·B S WilsonS Ferrone
Jan 1, 1984·Hybridoma·G M StuhlmillerH F Seigler
Dec 1, 1990·Hybridoma·T R ShockleyM L Yarmush
Jun 1, 1992·Hybridoma·J J StastnyC W Beattie
Dec 1, 1983·Journal of Cutaneous Pathology·P G NataliS Ferrone
Jun 1, 1982·Cancer Treatment Reviews·C S Foster
May 15, 1983·International Journal of Cancer. Journal International Du Cancer·D StramignoniJ Schlom
Jan 1, 1989·Seminars in Surgical Oncology·J H WongD L Morton
May 15, 1982·International Journal of Cancer. Journal International Du Cancer·H J GarriguesK E Hellström
May 15, 1982·International Journal of Cancer. Journal International Du Cancer·M NutiJ Schlom
Jan 15, 1986·International Journal of Cancer. Journal International Du Cancer·M D Bregman, F L Meyskens
Mar 15, 1982·International Journal of Cancer. Journal International Du Cancer·M Y YehK E Hellström
Apr 1, 1985·Pathology·P Hersey
Sep 27, 2013·PloS One·Zhuo TangHassan M Fathallah-Shaykh
Jan 1, 1987·Cancer Immunology, Immunotherapy : CII·G M StuhlmillerH F Seigler
Feb 1, 1983·Australian and New Zealand Journal of Medicine·P J Thurlow, I F McKenzie