Two insulin-like peptide family members from the mosquito Aedes aegypti exhibit differential biological and receptor binding activities.

Molecular and Cellular Endocrinology
Zhimou WenMark R Brown

Abstract

Insects encode multiple ILPs but only one homolog of the vertebrate IR that activates the insulin-signaling pathway. However, it remains unclear whether all insect ILPs are high affinity ligands for the IR or have similar biological functions. The yellow fever mosquito, Aedes aegypti, encodes eight ILPs with prior studies strongly implicating ILPs from the brain in regulating metabolism and the maturation of eggs following blood feeding. Here we addressed whether two ILP family members expressed in the brain, ILP4 and ILP3, have overlapping functional and receptor binding activities. Our results indicated that ILP3 exhibits strong insulin-like activity by elevating carbohydrate and lipid storage in sugar-fed adult females, whereas ILP4 does not. In contrast, both ILPs exhibited dose-dependent gonadotropic activity in blood-fed females as measured by the stimulation of ovaries to produce ecdysteroids and the uptake of yolk by primary oocytes. Binding studies using ovary membranes indicated that ILP4 and ILP3 do not cross compete; a finding further corroborated by cross-linking and immunoblotting experiments showing that ILP3 binds the MIR while ILP4 binds an unknown 55kDa membrane protein. In contrast, each ILP activated the ins...Continue Reading

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Citations

May 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lynn M Riddiford
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Jul 3, 2021·Insects·Patrycja SkowronekAneta Strachecka
Jul 17, 2021·Frontiers in Physiology·Szymon ChowańskiJoanna Pacholska-Bogalska

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