Two isocitrate dehydrogenases from a psychrophilic bacterium, Colwellia psychrerythraea

Extremophiles : Life Under Extreme Conditions
Shinsuke MakiYasuhiro Takada

Abstract

Two structurally different monomeric and dimeric types of isocitrate dehydrogenase (IDH; EC 1.1.1.42) isozymes were confirmed to exist in a psychrophilic bacterium, Colwellia psychrerythraea, by Western blot analysis and the genes encoding them were cloned and sequenced. Open reading frames of the genes (icd-M and icd-D) encoding the monomeric and dimeric IDHs of this bacterium, IDH-M and IDH-D, were 2,232 and 1,251 bp in length and corresponded to polypeptides composed of 743 and 416 amino acids, respectively. The deduced amino acid sequences of the IDH-M and IDH-D showed high homology with those of monomeric and dimeric IDHs from other bacteria, respectively. Although the two genes were located in tandem, icd-M then icd-D, on the chromosomal DNA, a Northern blot analysis and primer extension experiment revealed that they are transcribed independent of each other. The expression of the monomeric and dimeric IDH isozyme genes in C. maris, a psychrophilic bacterium of the same genus as C. psychrerythraea, is known to be induced by low temperature and acetate, respectively, but no such induction in the expression of the C. psychrerythraea icd-M and icd-D genes was detected. IDH-M and IDH-D overexpressed in Escherichia coli were p...Continue Reading

References

Jun 7, 1974·Biochimica Et Biophysica Acta·W F BurkeH C Reeves
Nov 1, 1972·Archives of Biochemistry and Biophysics·A E Chung, J E Braginski
Sep 1, 1974·Journal of Bacteriology·F C NeidhardtD F Smith
Aug 1, 1972·Journal of Bacteriology·J Y D'Aoust, D J Kushner
Dec 10, 1997·Biochimica Et Biophysica Acta·C GerdayG Feller
Mar 29, 2000·Journal of Molecular Biology·K MiyazakiF H Arnold
Feb 24, 2001·Archives of Biochemistry and Biophysics·R Chen, H Yang
May 3, 2002·European Journal of Biochemistry·Konstantinos MavromatisVassilis Bouriotis
Oct 22, 2003·FEMS Microbiology Letters·Zheng-Xiang WangAlexander Steinbüchel

Citations

Jun 16, 2010·Archives of Microbiology·Shougo MatsuoYasuhiro Takada
Jun 6, 2007·Current Opinion in Microbiology·Manuel FerrerPeter N Golyshin
Aug 19, 2014·International Journal of Medical Microbiology : IJMM·Anja EichnerMichael Hogardt
Apr 18, 2018·Astrobiology·Jacob HeinzDirk Schulze-Makuch
Aug 24, 2017·Environmental Microbiology·Isabelle Raymond-BouchardLyle G Whyte
Mar 13, 2018·FEMS Microbiology Ecology·Isabelle Raymond-BouchardLyle G Whyte

Related Concepts

Enzyme Stability
Isocitrate Dehydrogenase-I
Alloenzymes
Chimeric Proteins, Recombinant
Poly(A) Tail
Determination, Sequence Homology
Genome, Bacterial
Homologous Sequences, Amino Acid
Alteromonadaceae

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