Two isoforms of prostaglandin EP3 receptor exhibiting constitutive activity and agonist-dependent activity in Rho-mediated stress fiber formation

Biochemical and Biophysical Research Communications
H HasegawaA Ichikawa

Abstract

We have cloned two isoforms of the mouse prostaglandin E receptor EP3 subtype, EP3alpha and EP3beta, with different carboxyl-terminal tails, produced through alternative splicing. To determine the functional differences between the two isoforms, we examined the role of the isoforms in regulation of the actin cytoskeleton using Mardin-Darby canine kidney cells expressing these isoforms. The EP3alpha isoform constitutively induced stress fiber formation, independent of an agonist, while the EP3beta isoform agonist-dependently induced stress fiber formation. Pertussis toxin did not prevent stress fiber formation. This signaling pathway is mediated by Rho, because C3 transferase microinjection inhibited stress fiber formation. Therefore, the physiological significance of these isoforms of the EP3 receptor may lie in their different agonist dependency in Rho-mediated stress fiber formation via a pertussis toxin-insensitive G protein.

References

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Citations

Oct 23, 2012·Molecular Pharmacology·Chandramohan NatarajanRichard M Breyer
Oct 5, 2001·Trends in Pharmacological Sciences·G Milligan, J H White
Oct 8, 2009·Prostaglandins & Other Lipid Mediators·Katrin Andreasson
Aug 19, 2003·British Journal of Pharmacology·Winnie W C ShumYasuharu Sasaki
Sep 9, 2016·Angewandte Chemie·Gisbert SchneiderKarl-Heinz Altmann
Dec 19, 2006·The Journal of Biological Chemistry·Keisuke YanagidaTakao Shimizu
Feb 28, 1998·The Journal of Biological Chemistry·H KatohM Negishi
Jan 31, 2008·The Journal of Biological Chemistry·Ines M Macias-PerezAmbra Pozzi
Jul 29, 2004·The Journal of Biological Chemistry·Naoka MurakamiTakao Shimizu
Aug 23, 2002·European Journal of Pharmacology·Rüdiger BlindtJutta Meyer-Kirchrath

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