Abstract
We have cloned two isoforms of the mouse prostaglandin E receptor EP3 subtype, EP3alpha and EP3beta, with different carboxyl-terminal tails, produced through alternative splicing. To determine the functional differences between the two isoforms, we examined the role of the isoforms in regulation of the actin cytoskeleton using Mardin-Darby canine kidney cells expressing these isoforms. The EP3alpha isoform constitutively induced stress fiber formation, independent of an agonist, while the EP3beta isoform agonist-dependently induced stress fiber formation. Pertussis toxin did not prevent stress fiber formation. This signaling pathway is mediated by Rho, because C3 transferase microinjection inhibited stress fiber formation. Therefore, the physiological significance of these isoforms of the EP3 receptor may lie in their different agonist dependency in Rho-mediated stress fiber formation via a pertussis toxin-insensitive G protein.
References
Aug 7, 1992·Cell·A J Ridley, A Hall
May 1, 1988·Gastroenterology·M C ChenA H Soll
Sep 1, 1984·The Journal of Endocrinology·J F KrallS G Korenman
Jul 1, 1984·The Journal of Clinical Investigation·A Garcia-Perez, W L Smith
Oct 26, 1995·Biochimica Et Biophysica Acta·M NegishiA Ichikawa
Oct 20, 1995·The Journal of Biological Chemistry·A M BuhlG L Johnson
Jan 1, 1994·Annual Review of Cell Biology·A Hall
Sep 9, 1993·Nature·T NambaS Narumiya
Jan 26, 1996·The Journal of Biological Chemistry·H HasegawaA Ichikawa
Nov 22, 1996·The Journal of Biological Chemistry·H KatohA Ichikawa
Citations
Mar 4, 2000·The Biochemical Journal·H FujitaM Negishi
Oct 23, 2012·Molecular Pharmacology·Chandramohan NatarajanRichard M Breyer
Jul 13, 2004·Gut·Y ShojiK Wakabayashi
Oct 5, 2001·Trends in Pharmacological Sciences·G Milligan, J H White
Feb 19, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Raymond B Penn
Oct 8, 2009·Prostaglandins & Other Lipid Mediators·Katrin Andreasson
May 13, 2008·Neuroscience Letters·Jun LiKatrin Andreasson
Aug 19, 2003·British Journal of Pharmacology·Winnie W C ShumYasuharu Sasaki
Sep 9, 2016·Angewandte Chemie·Gisbert SchneiderKarl-Heinz Altmann
Dec 28, 1999·Protein Engineering·L OliveiraG Vriend
May 10, 2000·FEBS Letters·H HasegawaM Negishi
Dec 19, 2006·The Journal of Biological Chemistry·Keisuke YanagidaTakao Shimizu
Jul 15, 2011·Pharmacological Reviews·D F WoodwardS Narumiya
Jul 20, 1999·The Journal of Biological Chemistry·H HasegawaM Negishi
Feb 28, 1998·The Journal of Biological Chemistry·H KatohM Negishi
Jan 18, 2005·The Biochemical Journal·Andrea Pathe-Neuschäfer-RubeGerhard P Püschel
Jan 31, 2008·The Journal of Biological Chemistry·Ines M Macias-PerezAmbra Pozzi
Dec 10, 1999·The Journal of Biological Chemistry·K L PierceJ W Regan
Jul 29, 2004·The Journal of Biological Chemistry·Naoka MurakamiTakao Shimizu
Sep 24, 2020·Pharmacological Reviews·Xavier NorelLucie H Clapp
Aug 23, 2002·European Journal of Pharmacology·Rüdiger BlindtJutta Meyer-Kirchrath
Jun 28, 2003·Journal of Cell Science·Grazia TammaEnno Klussmann