PMID: 9530873Apr 8, 1998Paper

Two jasmonate-inducible myrosinase-binding proteins from Brassica napus L. seedlings with homology to jacalin

Planta
N Geshi, A Brandt

Abstract

Two homologous but different cDNAs encoding a 97-kDa and a 70-kDa protein from Brassica napus L. seedlings have been characterized. Both proteins contain sequence motifs with high homology to the IgA binding lectin, jacalin, and the deduced 97-kDa protein contains the peptide sequences of myrosinase-binding protein. The 70-kDa and the 97-kDa protein can both be isolated as a complex containing myrosinase, indicating they indeed are myrosinase-binding proteins. We provide evidence that the 70-kDa protein binds IgA in vitro, and therefore classify the protein as a jacalin-type lectin. Both the 97-kDa and the 70-kDa proteins are encoded by a small number of genes in the Brassica genome. The mRNA for the 97-kDa protein is detected in both light- and dark-grown seedlings, whereas the mRNA for the 70-kDa protein is mainly detected in etiolated seedlings. The transcript levels for both proteins are transient and are rapidly increased by methyl jasmonate. The 70-kDa protein is synthesized de novo during germination and accumulates mainly in the hypocotyl and in the root. By immunogold labeling we show that a few cells scattered in the cotyledons of young seedlings (approx. 5% of total cells), contain protein-body-like structures with t...Continue Reading

Citations

Nov 18, 2000·Electrophoresis·T A PrimeP Dupree
Feb 14, 2007·Plant Cell Reports·Choonkyun JungJong-Joo Cheong
Jan 10, 2013·Glycoconjugate Journal·Maria A SouzaMaria Cristina Roque-Barreira
Sep 18, 2012·Applied Biochemistry and Biotechnology·Venkidasamy BaskarSe Won Park
Apr 23, 2003·Biochemical and Biophysical Research Communications·Pierre RougéEls J M Van Damme
Feb 20, 2009·The Journal of Biological Chemistry·Ralph Kissen, Atle M Bones
Jul 12, 2007·Plant & Cell Physiology·Nausicaä LannooEls J M Van Damme
Dec 22, 2006·Genes & Development·Ramya RajagopalanDavid P Bartel
Jan 1, 2010·The Arabidopsis Book·Ute Wittstock, Meike Burow
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·S T ChisholmJ C Carrington
Aug 4, 2009·Biochimica Et Biophysica Acta·Nausicaä Lannoo, Els J M Van Damme
May 13, 2008·Phytochemistry·Anne Vinther MorantSøren Bak
Dec 28, 2007·Journal of Biochemical and Biophysical Methods·Natalia BellostasHilmer Sørensen
Jan 22, 2005·The Plant Journal : for Cell and Molecular Biology·Jane GillespieJohn C Rogers
Oct 7, 2004·Trends in Plant Science·Els J M Van DammeWilly J Peumans
Aug 4, 2006·Phytochemistry·Nina TerpIvo Feussner
Feb 23, 2002·Protein Expression and Purification·Frauke V Härtel, Anders Brandt
Nov 9, 2016·Frontiers in Plant Science·Jianwei GuMaoteng Li
Jun 6, 2019·Frontiers in Plant Science·Shweta ChhajedSixue Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.