Two K(+)-selective conductances in single proximal tubule cells isolated from frog kidney are regulated by ATP

The Journal of Physiology
L Robson, M Hunter

Abstract

1. The whole-cell and single channel patch clamp techniques were used to identify K(+)-selective conductances in single proximal tubule cells isolated from frog kidney and to examine their ATP sensitivity. Whole-cell currents were inhibited by the K+ channel inhibitors Ba2+ and quinidine in a dose-dependent manner. Addition of Ba2+ alone, quinidine alone, or both inhibitors together revealed two separate conductances, one of which was blocked by both Ba2+ and quinidine (GBa)1, the other being sensitive to quinidine alone (Gquin). 2. With Na(+)-containing Ringer solution in the bath and K(+)-containing Ringer solution in the pipette, both currents were selective for K+ over Na+. The K+ : Na+ selectivity ratio of GBa was around 50:1, while that of Gquin was 4:1. In symmetrical KCl solutions GBa showed inward rectification, while Gquin demonstrated outward rectification. 3. In the absence of pipette ATP, both GBa and Gquin ran down over 10 min. However, when 2 mM ATP was included in the pipette GBa increased, while Gquin remained unchanged. 4. Single channel studies demonstrated that a basolateral K+ channel shared several of the characteristics of GBa. It was inhibited by both Ba2+ and quinidine, underwent run-down in excised pat...Continue Reading

Citations

Nov 23, 2013·Purinergic Signalling·Geoffrey BurnstockMatthew A Bailey
Aug 6, 2000·British Journal of Pharmacology·R EngbersenF G Russel
Feb 8, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jelena Nesovic-OstojicJovica Milovanovic
Feb 16, 2002·The Japanese Journal of Physiology·J F NoulinJ Y Lapointe
Dec 30, 2014·Physiological Reviews·Francisco V SepúlvedaMaría Isabel Niemeyer
Apr 9, 2001·American Journal of Physiology. Renal Physiology·I D Millar, L Robson

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