PMID: 2029638Mar 1, 1991

Two kynurenine aminotransferases in human brain

Brain Research
E OkunoRobert Schwarcz

Abstract

Using Tris-acetate buffer rather than conventional phosphate buffer, it was possible to detect two distinct proteins capable of producing the neuroinhibitory brain metabolite kynurenic acid (KYNA) from L-kynurenine in human brain tissue. The two kynurenine aminotransferases (KATs), arbitrarily termed 'KAT I' and 'KAT II', could be physically separated by isoelectric focussing on a pH 3-10 Ampholine gradient, and, more completely, by differential elution from a DEAE-Sepharose column. KAT I showed a pronounced preference for pyruvate as a co-factor and had a pH optimum of 9.6. In contrast, KAT II was virtually equally active when either pyruvate or 2-oxoglutarate were used as the aminoacceptor, and its pH optimum was 7.4. Moreover, KAT I and KAT II differed with regard to their sensitivity to amino acids and as the aminoacceptor, and its pH optimum was 7.4. Moreover, KAT I and KAT II differed with regard to their sensitivity to amino acids and kinetic characteristics. The existence of two separate enzymes capable of producing KYNA in the human brain raises the question if and to what extent each of the enzymes regulates the cerebral synthesis of KYNA and its possible role as a modulator of excitatory amino acid receptor function.

References

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Citations

May 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·T Noguchi, R Kido
Apr 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·I HaradaR Kido
Jan 1, 1978·Biochemical Society Transactions·M H JosephA M Lawson
Jan 20, 1975·Biochemical and Biophysical Research Communications·M C Tobes, M Mason
Jul 1, 1990·Trends in Pharmacological Sciences·G L Collingridge, W Singer
Oct 1, 1990·Journal of the Neurological Sciences·A VezzaniRobert Schwarcz
Mar 1, 1989·Journal of Neurochemistry·J H ConnickT W Stone
May 1, 1989·Journal of Neurochemistry·Waldemar A TurskiRobert Schwarcz
Feb 1, 1987·Neurochemical Pathology·W Raabe
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Nov 1, 1974·The Biochemical Journal·M NakataniR Kido
Jul 1, 1974·Journal of Neurochemistry·Y MinatogawaR Kido
Jul 2, 1984·Life Sciences·Robert SchwarczC Köhler

Related Concepts

OGDH
Desmolases
Brain
Transaminases
Kynurenine aminotransferase II
Isoelectric Focusing
Dichlorovinylcysteine beta-lyase
Hydrogen-Ion Concentration

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