Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli

Journal of General Microbiology
R J KadnerC M Stephens


The Escherichia coli uhp T gene encodes an active transport system for sugar phosphates. When the uhp T gene was carried on a multicopy plasmid, amplified levels of transport activity occurred, and growth of these strains was inhibited upon the addition of various sugar phosphates. Two different mechanisms for this growth inhibition were distinguished. Exposure to glucose-6-phosphate, fructose-6-phosphate or mannose-6-phosphate, which enter directly into the glycolytic pathway, resulted in cessation of growth and substantial loss of viability. Cell killing was correlated with the production of the toxic metabolite, methylglyoxal. In contrast, addition of 2-deoxyglucose-6-phosphate, galactose-6-phosphate, glucosamine-6-phosphate or arabinose-5-phosphate, which do not directly enter the glycolytic pathway, resulted in growth inhibition without engendering methylglyoxal production or cell death. Inhibition of growth could result from excessive accumulation of organophosphates in the cell or depletion of inorganic phosphate pools as a result of the sugar-P/Pi exchange process catalysed by UhpT. The phosphate-dependent uptake of glycerol-3-phosphate by the GlpT antiporter was strongly inhibited under conditions of elevated sugar-pho...Continue Reading


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Related Concepts

fructose-6-phosphate, magnesium (1: 1) salt, (D)-isomer
mannose-6-phosphate sodium salt, (D)-isomer
arabinose 5-phosphate, di-Li salt
alpha-glycerophosphoric acid, monomagnesium salt
galactose-6-phosphate, monosodium salt (1:1), (D)-isomer
UhpT protein, E coli
Bacterial Proteins
Carrier Proteins
Cell Division Phases

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