Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli

Journal of General Microbiology
R J KadnerC M Stephens

Abstract

The Escherichia coli uhp T gene encodes an active transport system for sugar phosphates. When the uhp T gene was carried on a multicopy plasmid, amplified levels of transport activity occurred, and growth of these strains was inhibited upon the addition of various sugar phosphates. Two different mechanisms for this growth inhibition were distinguished. Exposure to glucose-6-phosphate, fructose-6-phosphate or mannose-6-phosphate, which enter directly into the glycolytic pathway, resulted in cessation of growth and substantial loss of viability. Cell killing was correlated with the production of the toxic metabolite, methylglyoxal. In contrast, addition of 2-deoxyglucose-6-phosphate, galactose-6-phosphate, glucosamine-6-phosphate or arabinose-5-phosphate, which do not directly enter the glycolytic pathway, resulted in growth inhibition without engendering methylglyoxal production or cell death. Inhibition of growth could result from excessive accumulation of organophosphates in the cell or depletion of inorganic phosphate pools as a result of the sugar-P/Pi exchange process catalysed by UhpT. The phosphate-dependent uptake of glycerol-3-phosphate by the GlpT antiporter was strongly inhibited under conditions of elevated sugar-pho...Continue Reading

Citations

Feb 14, 2003·FEMS Microbiology Letters·Anne W GrantElizabeth M Ellis
Sep 25, 1998·Trends in Microbiology·P Gonçalves, R J Planta
Jan 20, 2011·Nucleic Acids Research·Jennifer B Rice, Carin K Vanderpool
Jan 11, 2012·Antimicrobial Agents and Chemotherapy·Santiago Ramón-GarcíaJosé A Aínsa
Dec 20, 2011·Applied and Environmental Microbiology·Patrick K H LeeLisa Alvarez-Cohen
Mar 14, 2000·Journal of Bacteriology·P L HuynhR Brückner
Dec 4, 2004·Journal of Bacteriology·Lynn C ThomasonJudah L Rosner
Nov 14, 2013·Journal of Mammary Gland Biology and Neoplasia·Feng-Qi Zhao
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Caryn S Wadler, Carin K Vanderpool
Sep 24, 2014·Current Microbiology·Xue YanVianney Pichereau
Jul 13, 2000·Critical Reviews in Biotechnology·J C Diaz Ricci, M E Hernández
Aug 17, 2011·Biochimica Et Biophysica Acta·Gregory R Richards, Carin K Vanderpool
Feb 16, 2016·Biochemistry. Biokhimii︠a︡·O V KosmachevskayaA F Topunov
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·David KentnerDirk Bumann
Mar 27, 2007·Current Opinion in Microbiology·Carin K Vanderpool
Aug 13, 2004·Molecular Microbiology·Hitoshi KomatsuzawaMotoyuki Sugai
Nov 8, 2014·IUBMB Life·Sangeeta ChakrabortyDipshikha Chakravortty
Sep 16, 2015·ACS Synthetic Biology·Justin R KlesmithTimothy A Whitehead
Feb 12, 2014·Current Opinion in Microbiology·Ake Västermark, Milton H Saier
Sep 24, 2009·Proteome Science·Svenja LüdersEzequiel Franco-Lara
Apr 19, 2011·Biochimica Et Biophysica Acta·Tomoko Oya-ItoToshikazu Yoshikawa
Aug 1, 2014·Frontiers in Cellular and Infection Microbiology·Kai Papenfort, Jörg Vogel
Sep 11, 2013·Annual Review of Genetics·Maksym Bobrovskyy, Carin K Vanderpool
Mar 25, 2018·Scientific Reports·Rafael BaptistaLuis A J Mur
Jan 30, 2018·Microbial Biotechnology·Kees C H van der ArkClara Belzer
Feb 16, 2018·Molecular Biology of the Cell·Patrick A GibneyDavid Botstein
Nov 22, 2017·BMC Systems Biology·Víctor A López-AgudeloRigoberto Ríos-Estepa
Mar 7, 1998·Molecular Microbiology·S TötemeyerI R Booth
Nov 5, 2017·Biochemical Society Transactions·Erik Holmqvist, E Gerhart H Wagner
Oct 8, 2017·Applied and Environmental Microbiology·Julie R KesslerGregory R Richards
Sep 3, 2013·Journal of Bacteriology·Gregory R RichardsCarin K Vanderpool
Jan 18, 2017·The Journal of Antimicrobial Chemotherapy·M Ballestero-TéllezA Pascual
Aug 21, 2018·Biotechnology for Biofuels·Carlos H Luna-FloresEsteban Marcellin
Mar 21, 2020·Nature Communications·Qinqin ZhaoZixin Deng
Dec 23, 2016·Metabolomics : Official Journal of the Metabolomic Society·Stefan WeidtKarl E V Burgess
Mar 10, 2021·Journal of Industrial Microbiology & Biotechnology·K T Shanmugam, Lonnie O Ingram

Related Concepts

2-deoxyglucose-6-phosphate
fructose-6-phosphate, magnesium (1: 1) salt, (D)-isomer
mannose-6-phosphate sodium salt, (D)-isomer
arabinose 5-phosphate, di-Li salt
alpha-glycerophosphoric acid, monomagnesium salt
galactose-6-phosphate, monosodium salt (1:1), (D)-isomer
UhpT protein, E coli
Bacterial Proteins
Carrier Proteins
Cell Division Phases

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Laryngeal Neoplasms

Laryngeal Neoplasms occur in the Larynx and are typically associated with smoking and alcohol consumption. Discover the latest research on Laryngeal Neoplasms here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.