PMID: 9639603Jun 26, 1998Paper

Two membrane anchors of Wolinella succinogenes hydrogenase and their function in fumarate and polysulfide respiration

Archives of Microbiology
Roland GrossA Kröger

Abstract

Wolinella succinogenes can grow by anaerobic respiration with fumarate or polysulfide as the terminal electron acceptor, and H2 or formate as the electron donor. A DeltahydABC mutant lacking the hydrogenase structural genes did not grow with H2 and either fumarate or polysulfide. In contrast to the wild-type strain, the mutant grown with fumarate and with formate instead of H2 did not catalyze the reduction of fumarate, polysulfide, dimethylnaphthoquinone, or benzyl viologen by H2. Growth and enzymic activities were restored upon integration of a plasmid carrying hydABC into the genome of the DeltahydABC mutant. The DeltahydABC mutant was complemented with hydABC operons modified by artificial stop codons in hydA (StopA) or at the 5'-end of hydC (StopC). The StopC mutant lacked HydC, and the hydrophobic C-terminus of HydA was missing in the hydrogenase of the StopA mutant. The two mutants catalyzed benzyl viologen reduction by H2. The enzyme activity was located in the membrane of the mutants. A mutant with both modifications (StopAC) contained the activity in the periplasm. The three mutants did not grow with H2 and either fumarate or polysulfide, and did not catalyze dimethylnaphthoquinone reduction by H2. We conclude that th...Continue Reading

Citations

Jan 17, 2004·Microbial Pathogenesis·Stéphane BenoitRobert J Maier
Jan 23, 2002·Biochimica Et Biophysica Acta·Achim KrögerC Roy D Lancaster
Aug 8, 2002·FEMS Microbiology Reviews·Jörg Simon
Nov 1, 2003·FEMS Microbiology Letters·Roland Gross, Jörg Simon
Oct 26, 1999·The International Journal of Biochemistry & Cell Biology·M A SmithS L Hazell
Apr 4, 2002·Molecular Microbiology·Nicola R StanleyBen C Berks
Sep 27, 2000·Journal of Bacteriology·R UllmannA Kröger
Sep 29, 2011·Biotechnology for Biofuels·Aino-Maija LakaniemiJaakko A Puhakka
Feb 24, 2016·Frontiers in Microbiology·Moritz Hansen, Mirjam Perner
Aug 28, 2003·Molecular Microbiology·Kostas HatzixanthisFrank Sargent
Apr 2, 2008·Annals of the New York Academy of Sciences·Stéphane L Benoit, Robert J Maier
May 17, 2012·Biochimica Et Biophysica Acta·Maria-Eirini PandeliaWolfgang Nitschke
Jul 25, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Xiaojun LuoElisabeth Lojou
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
Jan 31, 2020·Microbiology and Molecular Biology Reviews : MMBR·Stéphane L BenoitChris Greening
Jan 6, 2021·Chemical Reviews·Filipa CalistoManuela M Pereira
May 5, 1999·Current Opinion in Chemical Biology·M J Maroney

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