Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle

Genes & Development
Miguel Godinho Ferreira, Julia Promisel Cooper

Abstract

Several considerations suggest that levels of the two major modes of double-strand break (DSB) repair, homologous recombination (HR), and nonhomologous end joining (NHEJ), are regulated through the cell cycle. However, this idea has not been explicitly tested. In the absence of the telomere-binding protein Taz1, fission yeast undergo lethal telomere fusions via NHEJ. These fusions occur only during periods of nitrogen starvation and fail to accumulate during logarithmic growth, when the majority of cells are in G2. We show that G1 arrest is the specific nitrogen starvation-induced event that promotes NHEJ between taz1(-) telomeres. Furthermore, the general levels of NHEJ and HR are reciprocally regulated through the cell cycle, so that NHEJ is 10-fold higher in early G1 than in other cell cycle stages; the reverse is true for HR. Whereas NHEJ is known to be dispensable for survival of DSBs in cycling cells, we find that it is critical for repair and survival of DSBs arising during G1.

References

Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T L Orr-WeaverR J Rothstein
Nov 19, 1993·Cell·L L Sandell, V A Zakian
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E Moynahan, M Jasin
Feb 26, 1998·Cell·B van SteenselT de Lange
Oct 17, 1998·Science·T M NakamuraT R Cech
Feb 26, 1999·Science·J KarlsederT de Lange
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·C H HaeringT R Cech
Oct 12, 2000·Molecular Biology of the Cell·P Baumann, T R Cech
Dec 6, 2000·Mutation Research· van den Bosch MA Pastink
Mar 21, 2001·Nature Reviews. Genetics·D C van GentR Kanaar
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M LisbyU H Mortensen
Oct 31, 2001·Trends in Cell Biology·A J PierceM Jasin
May 23, 2002·Genes & Development·Thomas CaspariAntony M Carr
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Rolf Jessberger
Oct 4, 2002·Current Biology : CB·Agata SmogorzewskaTitia de Lange
Jan 21, 2004·Molecular Cell·Miguel Godinho FerreiraJulia Promisel Cooper

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Citations

Oct 29, 2009·Chromosoma·Agnieszka RupnikMuriel Grenon
Jul 25, 2013·Mutation Research·Anne Margriet HeijinkMarcel A T M van Vugt
Sep 11, 2010·Nature·Tiago CarneiroMiguel Godinho Ferreira
Jan 3, 2007·Nature Cell Biology·Miguel Godinho Ferreira
Jun 28, 2011·Nature Reviews. Cancer·Andrew J Deans, Stephen C West
Oct 17, 2007·Nature Reviews. Microbiology·Stewart Shuman, Michael S Glickman
Aug 16, 2011·Nature Structural & Molecular Biology·Xuefeng ChenGrzegorz Ira
Mar 22, 2005·Nature Structural & Molecular Biology·Chunling GongMichael S Glickman
Aug 27, 2005·Nucleic Acids Research·Sergio González-BarreraLuis Blanco
Feb 19, 2008·Genes & Development·Jideofor AniukwuStewart Shuman
Dec 8, 2009·Science·Titia de Lange
Jul 11, 2006·Annual Review of Genetics·Karel RihaDorothy E Shippen
May 10, 2007·PloS One·Lilian RicaudMarie-Hélène Montané
Nov 22, 2005·Genetics·Christine R PrestonWilliam R Engels
Sep 16, 2009·Genetics·Ling XuConstance I Nugent
Jun 3, 2010·Frontiers in Bioscience (Landmark Edition)·Lakxmi Subramanian, Toru M Nakamura
Jun 10, 2014·Nature Communications·Chen-Chun PaiTimothy C Humphrey
Aug 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Victoria MartínPaul Russell
Nov 5, 2010·Annual Review of Genetics·Devanshi Jain, Julia Promisel Cooper
Nov 16, 2011·Cellular and Molecular Life Sciences : CMLS·Karen FinnMuriel Grenon
Aug 5, 2010·FEBS Letters·Pierre-Marie Dehé, Julia Promisel Cooper
Nov 17, 2015·International Journal of Molecular Sciences·Byeong Mo KimYonggeun Hong
Oct 27, 2011·Seminars in Cell & Developmental Biology·Torben R Kasparek, Timothy C Humphrey
Jan 16, 2010·Biochemical and Biophysical Research Communications·Stanislava GunisovaLubomir Tomaska
Oct 4, 2008·DNA Repair·Keiko Yata, Fumiko Esashi
Sep 24, 2008·Trends in Genetics : TIG·Mitch McVey, Sang Eun Lee
Apr 15, 2008·Current Opinion in Genetics & Development·Ofer Rog, Julia Promisel Cooper
Mar 27, 2007·Biochimica Et Biophysica Acta·Barbara Zellinger, Karel Riha
Aug 13, 2005·The EMBO Journal·Benjamin Pardo, Stéphane Marcand

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