PMID: 6414177Sep 1, 1983Paper

Two molecular weight species of murine gamma interferon

Virology
E A Havell, G L Spitalny

Abstract

Concanavalin A (Con A) affinity chromatography was used to concentrate and partially purify a murine gamma interferon (MuIFN gamma) from large volumes of crude MuIFN gamma (specific activity (SA), 10(4) units/mg of protein) produced by mitogen-induced T lymphocytes. Elution of the MuIFN gamma from the immobilized Con A with alpha-methyl-D-mannoside resulted in a 13-fold purification. Further purification of the Con A-bound MuIFN gamma was accomplished by gel filtration chromatography (SA 10(6.7) units/mg of protein) which also demonstrated the molecular weight (MW) to be 38,000 +/- 3000. Molecular weight determinations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, under both reducing and nonreducing conditions, revealed that the Con A-bound MuIFN gamma may exist as a dimer, consisting of monomers of 20,500 MW. Specific rabbit antiserum raised against the Con A-bound MuIFN gamma neutralized the antiviral activities of crude, Con A-bound and unbound MuIFN gamma fractions, as well as the 20,500 MW MuIFN gamma.

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A HavellJ Vilcek
Jan 1, 1983·Journal of Interferon Research·E A Havell, G L Spitalny

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Citations

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S MatsushitaD H Katz
Jan 1, 1986·Preparative Biochemistry·M YasudaN K Day
Jan 1, 1984·Archives of Virology·E A Havell, G L Spitalny
Jul 9, 2014·Molecular Biology Reports·Meetul KumarShashi Bala Singh

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