PMID: 7026687Jan 1, 1981Paper

Two molecularly independent surface receptors identify bovine T lymphocytes

Journal of Immunological Methods
W R Usinger, G A Splitter

Abstract

E rosette formation is a commonly used technique to identify T lymphocytes in many species; however, treatment of bovine E rosettes with rhodamine-conjugated F(ab')2 anti-immunoglobulin resulted in 10% (range 3-18%) of these cells exhibiting fluorescence. The failure of E rosettes to identify only non-immunoglobulin bearing cells suggested that an alternative method for T lymphocyte identification was essential. Using a labeled heterologous T cell antiserum and peanut agglutinin (PNA), identical populations of bovine T lymphocytes were identified. Unique and molecularly independent cell surface receptors were detected by dual fluorescent staining experiments differential inhibition of cellular binding of PNA by its carbohydrate ligand and capping experiments. Moreover, both reagents bound to approximately 62% of peripheral blood lymphocytes (PBLs) and virtually all thymocytes. Use of either T cell reagent in combination with rhodamine-conjugated F(ab')2 anti-bovine immunoglobulin provided a rapid, simple and reliable method for simultaneous enumeration of bovine T and B cells and permitted the detection of rare cells (less than 1%) expressing both T and B cell markers. Approximately 90% of all PBLs were identified as either T o...Continue Reading

References

Apr 1, 1979·Journal of Clinical Psychology·W T Tsushima, W S Towne
Oct 1, 1977·European Journal of Immunology·L MorettaM D Cooper
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y ReisnerB Ramot
Jan 1, 1979·Journal of Immunological Methods·T W PearsonK S Mayor-Withey
Jan 3, 1977·JAMA : the Journal of the American Medical Association·M M Thaler
Jan 1, 1976·Journal of Immunological Methods·J W Goding
Nov 1, 1973·The Journal of Experimental Medicine·S HammarströmM L Dillner

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Citations

Jan 1, 1984·Developmental and Comparative Immunology·D A Higgins
Apr 1, 1983·Veterinary Immunology and Immunopathology·C Johansson, B Morein
Sep 1, 1984·Veterinary Immunology and Immunopathology·M Miller-Edge, G A Splitter
May 1, 1985·Veterinary Immunology and Immunopathology·H A LewinD Bernoco
Apr 1, 1986·Veterinary Immunology and Immunopathology·C R RossiV S Panangala
Sep 1, 1986·Veterinary Immunology and Immunopathology·M Miller-Edge, G Splitter
Feb 1, 1987·Veterinary Immunology and Immunopathology·G E DuhamelB I Osburn
Dec 1, 1989·Veterinary Immunology and Immunopathology·M J RomanoH A Lewin
Aug 1, 1989·Veterinary Immunology and Immunopathology·A M Nagi, L A Babiuk
Jul 1, 1982·Cellular Immunology·G A Splitter, K M Everlith
Sep 1, 1986·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·C FossumP Matsson
Nov 1, 1987·Veterinary Pathology·R D ZwahlenA Schneider
Feb 1, 1985·Infection and Immunity·C L Baldwin, A J Winter

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