Jan 1, 1988

Two monoclonal antibodies recognizing different epitopes of blood group N antigen

Archivum Immunologiae Et Therapiae Experimentalis
K WaśniowskaE Lisowska

Abstract

Two monoclonal antibodies, N/61 (IgG2b) and N/92 (IgG2a), reacting preferentially with blood group N antigen, were obtained by immunization of BALB/c mice with human red cell membrane glycophorins. The antibodies showed distinctly different specificities. N/61 reacted with an epitope with NH2-terminal Leu residue, its free amino group, and sialic acid residues as essential components. The antibody N/92 reacted equally well with untreated and desialylated N glycoprotein, but did not react with de-O-glycosylated antigen. It seemed to recognize an epitope including both blood group N-characteristic amino acid residues, namely the 1st Leu and 5th Glu. The antibodies also differed in pH-dependence. N/61 showed maximal activity at pH 6-7 with tendency to decrease at higher pH values, whereas N/92 was not or weakly active up to pH 7, and showed a strong increase of activity at pH range 7-8.

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Mentioned in this Paper

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
N Syndrome
Mice, Inbred BALB C
Binding Sites, Antibody
Glycoproteins
Antigenic Specificity
Sialic Acid Assay
IgG2B
Neuraminidase

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